Sabir Irfan Ali, Nabi Farhan, Manzoor Muhammad Aamir, Ullah Fazal, Saeed Muhammad, Hashem Abeer, Alkahtani Jawaher, Abd-Allah Elsayed Fathi, Qadir Muslim
South China Agricultural University (SCAU), Guangzhou, Guangdong, 510642, China.
Department of Botany, University of Swabi, Swabi, Pakistan.
BMC Plant Biol. 2025 May 13;25(1):628. doi: 10.1186/s12870-025-06475-0.
Chitinase, a key enzyme family within the pathogenesis-related (PR) protein, plays a crucial role in plant defense by degrading chitin, a major component of fungal cell walls. The HvCHT genes in barley are involved in responses to biotic and abiotic stresses, although their full range of functions is not yet fully understood.
In this study, we identified 24 potential HvCHT genes through a genome-wide analysis. The comparative synteny analysis showed conserved relationships between HvCHT genes and their homologs in Sorghum bicolor, Oryza sativa, and Arabidopsis thaliana. Chromosomal mapping, gene structure, characterization, protein motif analysis, and miRNA regulation were performed to gain insight into the genetic structures of these genes. Segmental duplication events observed in the HvCHT family suggest an important role in the evolutionary development of these genes. Additionally, cis-regulatory element analysis revealed the presence of light-responsive elements, and regulators for Abscisic acid, methyl jasmonate (MeJA), salicylic acid, and gibberellins, indicating potential involvement in stress responses. Transcriptomic data showed differential expression of HvCHT genes in response to salt stress, with distinct patterns observed in leaf and root tissues. Furthermore, the genes defensive responses to drought stress and Fusarium infection were characterized across multiple time points. Notably, qRT-PCR analysis confirmed the upregulation of HvCHT1, HvCHT4, and HvCHT17, highlighting their potential involvement in stress-related pathways.
These findings provide a comprehensive overview of the HvCHT genes role in barley defense mechanisms, underlining their regulatory functions in biotic and abiotic stressors. The results lay the groundwork for future functional studies on HvCHT genes, with the potential to enhance stress tolerance in crops.
Not applicable.
几丁质酶是病程相关（PR）蛋白中的一个关键酶家族，通过降解几丁质（真菌细胞壁的主要成分）在植物防御中发挥关键作用。大麦中的HvCHT基因参与生物和非生物胁迫反应，但其全部功能尚未完全明确。
在本研究中，我们通过全基因组分析鉴定出24个潜在的HvCHT基因。比较共线性分析表明，HvCHT基因与其在高粱、水稻和拟南芥中的同源基因之间存在保守关系。进行了染色体定位、基因结构、特征分析、蛋白质基序分析和miRNA调控研究，以深入了解这些基因的遗传结构。HvCHT家族中观察到的片段重复事件表明其在这些基因的进化发展中起重要作用。此外，顺式调控元件分析揭示了光响应元件以及脱落酸、茉莉酸甲酯（MeJA）、水杨酸和赤霉素的调控因子的存在，表明其可能参与胁迫反应。转录组数据显示HvCHT基因在盐胁迫下差异表达，在叶和根组织中观察到不同模式。此外，还在多个时间点对这些基因对干旱胁迫和镰刀菌感染的防御反应进行了表征。值得注意的是，qRT-PCR分析证实了HvCHT1、HvCHT4和HvCHT17的上调，突出了它们在胁迫相关途径中的潜在作用。
这些发现全面概述了HvCHT基因在大麦防御机制中的作用，强调了它们在生物和非生物胁迫中的调控功能。研究结果为未来对HvCHT基因的功能研究奠定了基础，有望提高作物的胁迫耐受性。
不适用。
