Shehab-El-Deen Mohamed, Ali Mohamed, Alolayan Ibrahim, Aljuaythin Abdullah, Alrauji Yasser, Aldobaib Soliman, Elnesr Shaaban S
Department of Animal and Poultry Production, College of Agriculture and Food, Qassim University, Buraydah 51452, Al-Qassim, Saudi Arabia.
Department of Animal Production, Faculty of Agriculture, Suez Canal University, Ismailia 41522, Egypt.
Animals (Basel). 2025 Apr 28;15(9):1242. doi: 10.3390/ani15091242.
This study aimed to investigate the effects of adding different levels of punicalagin or oleuropein to TRIS diluent on the quality of frozen-thawed semen from Najdi rams. Semen was diluted using TRIS-based diluter with 15% egg yolk (control group); supplemented with 0.1, 0.5, or 1 mg/100 mL punicalagin (in Experiment 1); or supplemented with 1, 2.5, or 5 mg/100 mL oleuropein (in Experiment 2). The collected semen was evaluated and cryopreserved, with the motility and concentration of sperm assessed using a CASA system. The results showed that the total motile spermatozoa (TMS), percentage of progressive motile spermatozoa (PMS), curvilinear velocity (VCL), rectilinear velocity, average path velocity (VAP), linearity coefficient, straightness index, minor defects, and sperm vitality were higher in the 0.1 mg/100 mL punicalagin group ( < 0.05) than in other groups. HOST% post-thawing was significantly higher in all punicalagin groups compared to the control group ( < 0.001). The percentages of PMS, TMS, VCL, minor defects, and sperm vitality were higher in the 1 mg/100 mL oleuropein group ( < 0.05) than in other groups. Oleuropein supplementation at 5 mg/100 mL decreased VAP in cooled sperms, while all levels increased VAP post-thawing. HOST-positive sperms% post-thawing was higher in all oleuropein-treated groups than the control group ( < 0.001). Moreover, oleuropein nonsignificantly increased the acrosome integrity in cooled sperms, while higher studied concentrations of oleuropein (2.5 and 5 mg/100 mL) decreased the acrosome integrity in frozen sperms. In conclusion, adding punicalagin (0.1 mg/100 mL) or oleuropein (1 mg/100 mL) to TRIS diluent improved the quality of frozen-thawed semen from rams.
本研究旨在探讨在TRIS稀释液中添加不同水平的石榴皮素或橄榄苦苷对纳吉迪公羊冻融精液质量的影响。精液用含15%蛋黄的TRIS基础稀释液进行稀释（对照组）；在实验1中添加0.1、0.5或1mg/100mL石榴皮素；或在实验2中添加1、2.5或5mg/100mL橄榄苦苷。对采集的精液进行评估并冷冻保存，使用计算机辅助精子分析（CASA）系统评估精子的活力和浓度。结果表明，0.1mg/100mL石榴皮素组的总活动精子数（TMS）、进行性活动精子百分比（PMS）、曲线速度（VCL）、直线速度、平均路径速度（VAP）、线性系数、直线度指数、微小缺陷和精子活力均高于其他组（P<0.05）。与对照组相比，所有石榴皮素组解冻后的HOST%均显著更高（P<0.001）。1mg/100mL橄榄苦苷组的PMS、TMS、VCL、微小缺陷和精子活力百分比高于其他组（P<0.05）。在冷却精子中，添加5mg/100mL橄榄苦苷会降低VAP，而所有水平的橄榄苦苷在解冻后均会增加VAP。与对照组相比，所有橄榄苦苷处理组解冻后的HOST阳性精子%均更高（P<0.001）。此外，橄榄苦苷在冷却精子中对顶体完整性有非显著性增加作用，而在冷冻精子中，较高研究浓度（2.5和5mg/100mL）的橄榄苦苷会降低顶体完整性。总之，在TRIS稀释液中添加石榴皮素（0.1mg/100mL）或橄榄苦苷（1mg/100mL）可提高公羊冻融精液的质量。
