An Hyo Jung, Song Dae Hyun, Kee Changwon, Cho Hyun-Kyung
Department of Pathology, Gyeongsang National University Changwon Hospital, School of Medicine, Gyeongsang National University, Changwon 51472, Republic of Korea.
Institute of Medical Science, School of Medicine, Gyeongsang National University, Jinju 52727, Republic of Korea.
Int J Mol Sci. 2025 Apr 29;26(9):4244. doi: 10.3390/ijms26094244.
This study aimed to determine the microRNA (miRNA) profile extracted from exosomes in plasma samples in pseudoexfoliation (PEX) glaucoma patients compared to controls. A blood sample (10 mL) was obtained after acquiring written informed consent. Exosome was extracted from each plasma sample using an Exoquick-TC kit. RNA sequencing was performed for each exosome sample. A bioinformatics study was conducted for miRNA-related pathways and targets. A total of 14 Korean subjects (7 with PEX glaucoma; 7 age-matched controls) were involved in the final study. In exosomes of PEX glaucoma participants, 330 mature miRNAs were detected. Among these, three miRNAs were significantly upregulated, including hsa-miR-92b-5p (fold change: 24.68), hsa-miR-744-5p (fold change: 2.49), and hsa-miR-148b-3p (fold change: 3.96). Sixty-six miRNAs were significantly downregulated in PEX glaucoma patients compared to the controls (all < 0.05). These significantly altered miRNAs (both upregulated and downregulated) were associated with the gene ontology (GO) category of neurogenesis (9.41%), which accounted for the largest proportion. The expression of exosomal microRNAs in plasma was significantly different between PEX glaucoma patients and the controls. This suggests their possible roles in the pathogenic mechanism and a good diagnostic marker for PEX glaucoma.
本研究旨在确定与对照组相比，剥脱性（PEX）青光眼患者血浆样本中外泌体提取的微小RNA（miRNA）谱。在获得书面知情同意后采集血样（10 mL）。使用Exoquick-TC试剂盒从每个血浆样本中提取外泌体。对每个外泌体样本进行RNA测序。对miRNA相关途径和靶点进行生物信息学研究。最终研究共纳入14名韩国受试者（7例PEX青光眼患者；7例年龄匹配的对照）。在PEX青光眼参与者的外泌体中，检测到330种成熟miRNA。其中，三种miRNA显著上调，包括hsa-miR-92b-5p（倍数变化：24.68）、hsa-miR-744-5p（倍数变化：2.49）和hsa-miR-148b-3p（倍数变化：3.96）。与对照组相比，PEX青光眼患者中有66种miRNA显著下调（均<0.05）。这些显著改变的miRNA（上调和下调）与神经发生的基因本体（GO）类别相关（9.41%），占比最大。PEX青光眼患者和对照组血浆中外泌体微小RNA的表达存在显著差异。这表明它们在致病机制中可能发挥的作用，以及作为PEX青光眼良好诊断标志物的潜力。
