Niemczyk Wojciech, Kępa Małgorzata, Żurek Jacek, Aboud Ali, Skaba Dariusz, Wiench Rafał
Department of Periodontal Diseases and Oral Mucosa Diseases, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, Pl. Traugutta 2, 41-800 Zabrze, Poland.
Medical Center of Innovation, Wroclaw Medical University, Krakowska 26, 50-425 Wroclaw, Poland.
Int J Mol Sci. 2025 May 1;26(9):4303. doi: 10.3390/ijms26094303.
The rising prevalence of antibiotic resistance underscores the need for localized drug delivery systems that minimize systemic exposure. Autologous platelet concentrates (APCs), including concentrated platelet-rich fibrin (c-PRF) and liquid-phase concentrated growth factors (LPCGFs), have emerged as potential carriers for antimicrobial agents. This study aimed to evaluate the efficacy of c-PRF and LPCGF as carriers for three antibiotic formulations-amoxicillin with clavulanic acid, clindamycin, and a combination of amoxicillin with metronidazole-against methicillin-sensitive (MSSA), methicillin-resistant (MRSA), and (low-level natural resistance). The disk diffusion method was employed to incorporate antibiotics into both APC types, which were then applied to disks placed on bacterial cultures. The size of the inhibition zones was measured at 20-, 40-, 60-, and 80-h intervals. Every 20 h, the disks were transferred to a new Petri dish. Statistical analysis included Welch's -test and two-way ANOVA. c-PRF demonstrated superior performance as a carrier for amoxicillin and clindamycin, showing the presence of inhibition zones for up to 60 h. In contrast, LPCGF exhibited greater efficacy when used with the amoxicillin-metronidazole combination, particularly at higher concentrations. Both APCs showed limited effectiveness against when combined with clindamycin. The study confirms the suitability of autologous platelet concentrates as localized antibiotic delivery systems. The choice between c-PRF and LPCGF should be guided by the drug's physicochemical properties and clinical application. APCs offer a promising alternative for targeted antimicrobial therapy in dental and surgical settings.
抗生素耐药性的日益普遍凸显了对可将全身暴露降至最低的局部给药系统的需求。自体血小板浓缩物（APCs），包括浓缩富血小板纤维蛋白（c-PRF）和液相浓缩生长因子（LPCGFs），已成为抗菌剂的潜在载体。本研究旨在评估c-PRF和LPCGF作为三种抗生素制剂（阿莫西林克拉维酸、克林霉素以及阿莫西林与甲硝唑的组合）载体对甲氧西林敏感（MSSA）、耐甲氧西林（MRSA）以及（低水平天然耐药）的疗效。采用纸片扩散法将抗生素掺入两种类型的APC中，然后将其应用于放置在细菌培养物上的纸片。在20、40、60和80小时的间隔时间测量抑菌圈的大小。每20小时，将纸片转移到一个新的培养皿中。统计分析包括韦尔奇t检验和双向方差分析。c-PRF作为阿莫西林和克林霉素的载体表现出卓越性能，抑菌圈可持续存在长达60小时。相比之下，LPCGF与阿莫西林-甲硝唑组合使用时，尤其是在较高浓度下，表现出更高的疗效。当与克林霉素联合使用时，两种APC对的有效性均有限。该研究证实了自体血小板浓缩物作为局部抗生素给药系统的适用性。在c-PRF和LPCGF之间的选择应根据药物的物理化学性质和临床应用来指导。APCs为牙科和外科手术中的靶向抗菌治疗提供了一种有前景的替代方法。
