Górecki Mateusz, Żbikowska Aleksandra, Tokłowicz Małgorzata, Sajdak Stefan, Englert-Golon Monika, Andrusiewicz Mirosław
Department of Cell Biology, Poznan University of Medical Sciences, Rokietnicka 5D, 60-806 Poznań, Poland.
Cell Biology Research Group, Student Scientific Society, Poznan University of Medical Sciences, Rokietnicka 5E, 60-806 Poznań, Poland.
Int J Mol Sci. 2025 May 7;26(9):4461. doi: 10.3390/ijms26094461.
Ovarian cancer (OC) is a severe gynecological malignancy with a high mortality rate among women worldwide. It is often diagnosed at advanced stages due to the lack of effective screening methods. This study investigated the expression patterns of microRNAs (miRNAs) hsa-miR-21-5p and hsa-miR-145-5p as potential OC prognostic and diagnostic biomarkers and their correlation with estrogen-dependent ( & , and ) and hypoxia-neovascularization-induced (, , and ) pathway genes. Tissue samples obtained from twenty patients with confirmed ovarian cancer and twenty controls were analyzed using quantitative polymerase chain reaction (qPCR) to examine miRNA and mRNA levels. The qPCR analysis revealed significantly higher hsa-miR-21-5p and lower hsa-miR-145-5p expression in OC tissues than controls. Moreover, a significant trend was observed in hsa-miR-21-5p and hsa-miR-145-5p expression levels across normal, non-cancerous changes and malignant ovarian tissues. The hsa-miR-21-5p showed better diagnostic potential than hsa-miR-145-5p. We also observed inconsistent correlations in hsa-miR-21-5p and hsa-mir-145-5p and estrogen-related and hypoxia-neovascularization-dependent genes in ovarian cancer across all groups. This suggests that the relationship between these miRNAs and the selected genes is context-specific. Our findings suggest that hsa-miR-21-5p and hsa-miR-145-5p expression levels may be prognostic or diagnostic markers for ovarian cancer patients.
卵巢癌（OC）是一种严重的妇科恶性肿瘤，在全球女性中死亡率很高。由于缺乏有效的筛查方法，它常常在晚期才被诊断出来。本研究调查了微小RNA（miRNA）hsa-miR-21-5p和hsa-miR-145-5p作为潜在的OC预后和诊断生物标志物的表达模式，以及它们与雌激素依赖性（&、和）和缺氧-新血管生成诱导（、、和）途径基因的相关性。使用定量聚合酶链反应（qPCR）分析从20例确诊卵巢癌患者和20例对照中获得的组织样本，以检测miRNA和mRNA水平。qPCR分析显示，与对照组相比，OC组织中hsa-miR-21-5p表达显著更高，hsa-miR-145-5p表达更低。此外，在正常、非癌性变化和恶性卵巢组织中，观察到hsa-miR-21-5p和hsa-miR-145-5p表达水平有显著趋势。hsa-miR-21-5p显示出比hsa-miR-145-5p更好的诊断潜力。我们还观察到，在所有组中，卵巢癌中hsa-miR-21-5p和hsa-mir-145-5p与雌激素相关和缺氧-新血管生成依赖性基因之间的相关性不一致。这表明这些miRNA与所选基因之间的关系是上下文特异性的。我们的研究结果表明，hsa-miR-21-5p和hsa-miR-145-5p表达水平可能是卵巢癌患者的预后或诊断标志物。
