Fan Yuting, Yang Zhencong, Lin Xiao, Xu Zhoujin, Mu Lixia, Li Qingrong, Wu Xuli
School of Public Health, Health Science Center, Shenzhen University, Shenzhen 518060, China.
Sericulture and Agro-Processing Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Nutrients. 2025 May 6;17(9):1596. doi: 10.3390/nu17091596.
Hyperuricemia is a prevalent metabolic disorder characterized by elevated serum uric acid (UA) levels.
In this study, hydrolysate (SPP) derived from silkworm pupae protein was isolated and identified, demonstrating anti-hyperuricemic activity. The research aimed to investigate its anti-hyperuricemic and nephroprotective effects, along with potential mechanisms, through in vitro assays and in vivo experiments using potassium oxonate/hypoxanthine-induced hyperuricemic mice.
The SPP exhibited significant xanthine oxidase (XOD) inhibitory activity, with an IC value of 7.41 mg/mL. Furthermore, SPP administration effectively reduced serum UA, blood urea nitrogen (BUN), creatinine levels, and renal pro-inflammatory cytokines in hyperuricemic mice. Mechanistic studies revealed that the anti-hyperuricemic effects of SPP may involve XOD inhibition and the modulation of renal UA transporters, specifically upregulating organic anion transporter 1 (OAT1) and ATP-binding cassette subfamily G member 2 (ABCG2) expression. Histopathological analysis and inflammatory cytokine profiling further demonstrated that SPP alleviated renal inflammation and pathological damage.
These findings suggest that SPP possesses a notable urate-lowering efficacy and renal protective properties, highlighting its potential as a therapeutic agent for the management and prevention of hyperuricemia (HUA).
高尿酸血症是一种常见的代谢紊乱疾病，其特征是血清尿酸（UA）水平升高。
在本研究中，从蚕蛹蛋白中分离并鉴定出具有抗高尿酸血症活性的水解产物（SPP）。该研究旨在通过体外实验和使用氧嗪酸钾/次黄嘌呤诱导的高尿酸血症小鼠进行的体内实验，研究其抗高尿酸血症和肾保护作用以及潜在机制。
SPP表现出显著的黄嘌呤氧化酶（XOD）抑制活性，IC值为7.41mg/mL。此外，给予SPP可有效降低高尿酸血症小鼠的血清UA、血尿素氮（BUN）、肌酐水平以及肾脏促炎细胞因子。机制研究表明，SPP的抗高尿酸血症作用可能涉及XOD抑制和肾脏UA转运体的调节，特别是上调有机阴离子转运体1（OAT1）和ATP结合盒转运体G超家族成员2（ABCG2）的表达。组织病理学分析和炎症细胞因子分析进一步表明，SPP减轻了肾脏炎症和病理损伤。
这些发现表明，SPP具有显著的降尿酸功效和肾脏保护特性，突出了其作为治疗和预防高尿酸血症（HUA）治疗剂的潜力。
