Ren Chunguang, Liu Yu, Su Wenwen, Tian Bing
Guizhou Institute of Mountain Resources, Guiyang, Guizhou, China.
Guizhou Agricultural Ecology and Resource Protection Station, Agriculture and Rural Affairs Department of Guizhou Province, Guiyang, Guizhou, China.
Front Microbiol. 2025 Apr 29;16:1562366. doi: 10.3389/fmicb.2025.1562366. eCollection 2025.
Soft rot caused by is a serious fungal disease in kiwifruit observed during the storage period, and it seriously restricts the healthy and stable development of the kiwifruit industry. In the present study, the bacterial strain LMY3-5 with high antifungal activity was isolated from healthy kiwifruit tissues. Based on gyrA and 16S rRNA sequences, a phylogenetic tree was constructed, and LMY3-5 was identified as . The 16% cell-free supernatant (CFS) of LMY3-5 suppressed spore germination and mycelial growth by 97.32%. The 16% CFS of LMY3-5 could effectively inhibit the expansion of kiwifruit soft rot , and the inhibition rate was 73.59%. The scanning electron microscopy (SEM) observations of mycelia treated with the 16% CFS for 12 h showed that the mycelia were curved, wrinkled, and sunken. Moreover, transmission electron microscopy (TEM) observations revealed blurred boundaries of mycelial cell walls as well as plasmolysis and vacuolization. Propidium iodide (PI) staining showed that the CFS treatment damaged the cell membrane of and affected its permeability, which led to leakage of the nucleic acids and proteins. Simultaneously, a substantial increase in the activity of key enzymes (i.e., -1,3-glucanase and chitinase) was observed, which indicated damage to the cell wall function of GC/LC-MS analysis revealed the presence of 27 antimicrobial compounds. Thus, the LMY3-5 strain showed great potential as a biocontrol agent for soft rot disease in kiwifruit.
由[未提及的病原体]引起的软腐病是猕猴桃贮藏期一种严重的真菌病害，严重制约了猕猴桃产业的健康稳定发展。在本研究中，从健康的猕猴桃组织中分离出具有高抗真菌活性的细菌菌株LMY3 - 5。基于gyrA和16S rRNA序列构建了系统发育树，并将LMY3 - 5鉴定为[未提及的菌种]。LMY3 - 5的16%无细胞上清液（CFS）抑制[未提及的真菌]孢子萌发和菌丝生长的比例达97.32%。LMY3 - 5的16% CFS能有效抑制猕猴桃软腐病[未提及的病原菌]的扩展，抑制率为73.59%。用16% CFS处理[未提及的真菌]菌丝12小时后的扫描电子显微镜（SEM）观察表明，菌丝弯曲、起皱且凹陷。此外，透射电子显微镜（TEM）观察显示菌丝细胞壁边界模糊，同时出现质壁分离和液泡化。碘化丙啶（PI）染色表明CFS处理破坏了[未提及的真菌]的细胞膜并影响其通透性，导致核酸和蛋白质泄漏。同时，观察到关键酶（即β - 1,3 - 葡聚糖酶和几丁质酶）的活性大幅增加，这表明[未提及的真菌]细胞壁功能受损。气相色谱/质谱联用（GC/LC - MS）分析显示存在27种抗菌化合物。因此，LMY3 - 5菌株作为猕猴桃软腐病的生物防治剂具有巨大潜力。
