Wang Yiting, Ou Xichao, Zhao Bing, Xia Hui, Zheng Yang, Zhou Yang, Xing Ruida, Song Yuanyuan, Wang Shengfen, Zhao Yanlin, Zheng Huiwen
National Tuberculosis Reference Laboratory, Chinese Center for Disease Control and Prevention, Beijing, China.
Beijing Center for Disease Control and Prevention, Beijing Institute of Tuberculosis Control, Institute for Immunization and Prevention, Beijing Academy for Preventive Medicine, Beijing, China.
Front Microbiol. 2025 Apr 29;16:1573448. doi: 10.3389/fmicb.2025.1573448. eCollection 2025.
To analyze subtypes, microbiological characteristics and antimicrobial susceptibility of in China, a total of 153 isolates, collected from national drug resistance surveillance, were genotyped with whole genome sequencing and explored the antimicrobial susceptibility with broth microdilution. All isolates were classified as type I based on Average Nucleotide Identity(ANI). The 153 representatives were differentiated into 3 clusters with 141 genotypes, including 17 isolates from a cluster and 136 isolates with unique patterns. The EXS-1, EXS-3 and EXS-5 regions were involved in all isolates. Rifabutin and clarithromycin were the most highly active against strains, with the susceptible rate of 100 and 99.35%, respectively. Followed by amikacin and linezolid, the resistance rate was 5.88 and 7.19%, respectively. The resistance rate to rifampin (RIF) was 22.22%. As for the antibiotics without the breakpoint values, all isolates had very low MIC (0.03 μg/mL) and MIC (≤0.06 μg/mL) values against bedaquiline, sutezolid, delamanid, and clofazimine. Except for ciprofloxacin and moxifloxacin, the resistance rate of other drugs in cluster 3 was higher than that in cluster 1 and cluster 2. In conclusion, type I was the predominant genotype in China, and rifabutin and clarithromycin presented strong activities. The new drugs, used for the treatment of multidrug - resistant tuberculosis, have the potential to be potent agents in the treatment of infection. The clustering might contribute to the high resistance rate of .
为分析中国[具体细菌名称未给出]的亚型、微生物学特征及抗菌药物敏感性，从全国耐药监测中收集了153株[具体细菌名称未给出]分离株，采用全基因组测序进行基因分型，并通过肉汤微量稀释法探究抗菌药物敏感性。基于平均核苷酸同一性（ANI），所有分离株均被分类为I型。153株[具体细菌名称未给出]代表菌株被分为3个簇，有141种基因型，其中一个簇中有17株分离株，136株具有独特模式。所有分离株均涉及EXS - 1、EXS - 3和EXS - 5区域。利福布汀和克拉霉素对[具体细菌名称未给出]菌株活性最高，敏感率分别为100%和99.35%。其次是阿米卡星和利奈唑胺，耐药率分别为5.88%和7.19%。对利福平（RIF）的耐药率为22.22%。对于没有断点值的抗生素，所有分离株对贝达喹啉、舒替唑胺、地拉曼德和氯法齐明的最低抑菌浓度（MIC）（0.03μg/mL）和MIC（≤0.06μg/mL）值都非常低。除环丙沙星和莫西沙星外，簇3中其他药物的耐药率高于簇1和簇2。总之，I型是中国的主要基因型，利福布汀和克拉霉素呈现出较强活性。用于治疗耐多药结核病的新药有可能成为治疗[具体细菌名称未给出]感染的有效药物。聚类分析可能导致[具体细菌名称未给出]的高耐药率。
