Mehrparvar Tajoddini Malihe, Gheybi Elaheh, Rostami Mehdi, Mousavi Seyed Hadi, Hashemy Seyed Isaac, Rashidi Roghayeh, Soukhtanloo Mohammad
Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Avicenna J Phytomed. 2025 May-Jun;15(3):1082-1090. doi: 10.22038/ajp.2024.25214.
Destruction of dopaminergic neurons causes diseases. Various compounds with neuroprotective and antioxidant properties have been identified, including Hesperidin (HES) and Auraptene (AUR). We aimed in this study to evaluate the protective effects of these compounds in SH-SY5Y neuroblastoma cell line against the induced neurotoxicity of 6-hydroxydopamine (6-OHDA).
The MTT test to assess cell viability was used. Flow cytometry was conducted for the cell cycle analysis using propidium iodide (PI) stain. The intracellular production of reactive oxygen species (ROS) was assessed using 2, 7'-dichlorofluorescein diacetate (DCFDA) probe and fluorimetry.
Following 6-OHDA treatment, cell viability decreased, and G2/M arrest and ROS levels increased. Our intervention demonstrated that only HES has neuroprotective effects against 6-OHDA-induced toxicity.
HES protects SH-SY5Y cells against 6-OHDA-induced neural damage via inhibiting G2/M arrest, reducing the amount of ROS, and increasing cell viability. However, the different effects and more precise mechanisms are still unknown, and requires new research on animal and human models.
多巴胺能神经元的破坏会引发疾病。已鉴定出多种具有神经保护和抗氧化特性的化合物，包括橙皮苷（HES）和奥勒冈橘素（AUR）。在本研究中，我们旨在评估这些化合物对SH-SY5Y神经母细胞瘤细胞系免受6-羟基多巴胺（6-OHDA）诱导的神经毒性的保护作用。
采用MTT试验评估细胞活力。使用碘化丙啶（PI）染色通过流式细胞术进行细胞周期分析。使用2,7'-二氯荧光素二乙酸酯（DCFDA）探针和荧光法评估细胞内活性氧（ROS）的产生。
6-OHDA处理后，细胞活力下降，G2/M期阻滞和ROS水平升高。我们的干预表明，只有HES对6-OHDA诱导的毒性具有神经保护作用。
HES通过抑制G2/M期阻滞、减少ROS量和提高细胞活力来保护SH-SY5Y细胞免受6-OHDA诱导的神经损伤。然而，不同的作用和更精确的机制仍不清楚，需要在动物和人体模型上进行新的研究。
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