Synthesis of Marine-Inspired Multifaceted DNA Damaging Spirooxindoles Combating NSCLC and Associated Bacterial Infection.

Targeted therapeutics have gained prominence in combating non-small cell lung carcinoma (NSCLC) and opportunistic bacterial infections like (). This study explores dual-acting marine-inspired spirooxindoles to limit NSCLC and opportunistic bacteria. Pharmacophoric motifs from antitumor and antibacterial marine products were merged into a new series of pyrazole-clubbed spirooxindoles via a stereoselective [3 + 2] cycloaddition reaction. MTT screening identified , , and - as potent cytotoxic agents, with showing exceptional activity (IC = 0.042 μM) and tumor selectivity (SI = 58.28). exhibited antibacterial efficacy against (MIC = 25 μg/mL). DNA damage studies using a terbium(III) chloride biosensor revealed 's ability to damage both calf thymus and DNA at low concentrations. Docking simulations presumed that binds between DNA strands, while apoptosis studies indicated it induced G1/S phase cell cycle arrest and increased A549 apoptosis by 33.65%. These findings highlight as a promising lead for further studies.