Wang Chen, Chen Xinyu, Shen Zhen, Lv Jiaming, Yu Bobing, Xu Yansen, Tang Xiangming
College of Ecology and Applied Meteorology Nanjing University of Information Science & Technology Nanjing China.
Taihu Laboratory for Lake Ecosystem Research, State Key Laboratory of Lake Science and Environment Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences Nanjing China.
Ecol Evol. 2025 May 12;15(5):e71445. doi: 10.1002/ece3.71445. eCollection 2025 May.
The use of membrane filtration is currently the most common method for collecting bacteria in lake water. However, the impact of different filtration volumes on bacterial diversity and community composition in lake water remains unclear. In this study, we collected water samples from mesotrophic Lake Bosten and eutrophic Lake Taihu in China. For Lake Bosten, we employed six filtration volumes (100, 200, 400, 800, 1600, and 3200 mL), while for Lake Taihu, seven filtration volumes (100, 200, 300, 400, 500, 1000, and 2000 mL) were used. Subsequently, Illumina MiSeq was employed to sequence the 16S rRNA genes, and statistical analyses were conducted on bacterial communities. Our study revealed that the water filtration volume impacts bacterial diversity, community structure, and taxonomic composition in both lakes. Based on the comprehensive consideration of the research results, we recommend using a filtering volume of 400-800 mL in mesotrophic lakes and 200-400 mL in eutrophic lakes for bacterial community studies.
目前，膜过滤法是采集湖水中细菌最常用的方法。然而，不同过滤体积对湖水中细菌多样性和群落组成的影响仍不清楚。在本研究中，我们从中国的中营养博斯腾湖和富营养太湖采集了水样。对于博斯腾湖，我们采用了六个过滤体积（100、200、400、800、1600和3200毫升），而对于太湖，则使用了七个过滤体积（100、200、300、400、500、1000和2000毫升）。随后，使用Illumina MiSeq对16S rRNA基因进行测序，并对细菌群落进行统计分析。我们的研究表明，水过滤体积会影响两个湖泊中的细菌多样性、群落结构和分类组成。基于对研究结果的综合考虑，我们建议在中营养湖泊中进行细菌群落研究时使用400 - 800毫升的过滤体积，在富营养湖泊中使用200 - 400毫升的过滤体积。
