Zagumyonnaya Olga N, Zagumyonnyi Dmitry G, Gerasimova Elena A, Tikhonenkov Denis V
AquaBioSafe Laboratory, University of Tyumen, Tyumen, Russia.
Papanin Institute for Biology of Inland Waters, Russian Academy of Sciences, Borok, Russia.
Appl Environ Microbiol. 2025 Jun 18;91(6):e0033025. doi: 10.1128/aem.00330-25. Epub 2025 May 14.
The problem of increasing salinity and mineralization in natural and artificial freshwater bodies with climate warming is very relevant nowadays, as it leads to changes in the species composition of planktonic organisms. Testate amoebae are one of the responsive bioindicators that are sensitive to even minor changes in environmental conditions. In this study, a comparative analysis of the species diversity of planktonic testate amoebae was carried out in a number of lakes in the forest-steppe and forest-swamp natural zones of Western Siberia using microscopy and metabarcoding. One new species, sp. nov., was described. The detection frequency and the number of reads of amplicon sequence variants of potentially pathogenic testate amoebae belonging to the genera and were higher in forest-steppe lakes. Universal eukaryotic primers for the 18S rRNA gene are well suited for identifying testate amoebae from the supergroup Cercozoa but are practically not applicable for identifying Amoebozoa testaceans. The plankton of the lakes with the highest mineralization and salinity was characterized by the most specific species composition. These results should be taken into account when predicting changes in aquatic communities with further climate warming, which may also be associated with an increase in the occurrence of pathogenic testaceans that pose biosafety threats.IMPORTANCEMicroscopic and metabarcoding analyses reveal important differences in testate amoebae communities in lakes in two natural and climatic zones of Western Siberia that should be taken into account when predicting changes in aquatic communities with further climate warming, which may also be associated with an increase in the occurrence of pathogenic testaceans that pose biosafety threats.
随着气候变暖，天然和人工淡水水体盐度和矿化度增加的问题在当今非常突出，因为这会导致浮游生物的物种组成发生变化。有壳变形虫是对环境条件的微小变化都很敏感的响应性生物指示物种之一。在本研究中，利用显微镜检查和宏条形码技术，对西西伯利亚森林草原和森林沼泽自然区的多个湖泊中的浮游有壳变形虫的物种多样性进行了比较分析。描述了一个新物种， sp. nov.。属于 属和 属的潜在致病性有壳变形虫的扩增子序列变体的检测频率和读数数量在森林草原湖泊中更高。用于18S rRNA基因的通用真核引物非常适合鉴定来自Cercozoa超群的有壳变形虫，但实际上不适用于鉴定有壳的变形虫门。矿化度和盐度最高的湖泊中的浮游生物具有最独特的物种组成。在预测随着气候进一步变暖水生群落的变化时应考虑这些结果，这也可能与构成生物安全威胁的致病性有壳变形虫的出现增加有关。重要性显微镜和宏条形码分析揭示了西西伯利亚两个自然和气候区湖泊中有壳变形虫群落的重要差异，在预测随着气候进一步变暖水生群落的变化时应考虑这些差异，这也可能与构成生物安全威胁的致病性有壳变形虫的出现增加有关。
