Jiang Chuyan, Liang Yue, Ma Chao
Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, College of Horticulture, China Agricultural University, Beijing, China.
Methods Mol Biol. 2025;2916:17-24. doi: 10.1007/978-1-0716-4470-6_2.
Auxin plays a critical role in the inhibition of organ abscission. The changing of auxin gradient across the abscission zone (AZ) is the primary determinant of the abscission initiation and activation. To better understand the role of auxin, the distribution and content of auxin at different developmental stages of AZ need to be determined. This protocol provides a convenient and efficient procedure for precise localization of auxin in the petal AZ of rose (Rosa hybrida). It can be combined with plant hormone metabolomics to more accurately reflect changes in auxin during the abscission process. This method serves as a powerful tool for auxin detection and can be applied to broader plant hormone research.
生长素在抑制器官脱落中起关键作用。生长素在离层区（AZ）上梯度的变化是脱落起始和激活的主要决定因素。为了更好地理解生长素的作用，需要确定离层区在不同发育阶段生长素的分布和含量。本方案提供了一种便捷高效的程序，用于精确检测玫瑰（Rosa hybrida）花瓣离层区中生长素的定位。它可以与植物激素代谢组学相结合，以更准确地反映脱落过程中生长素的变化。该方法是生长素检测的有力工具，可应用于更广泛的植物激素研究。
