Vaissayre Virginie, Cazals Guillaume, Tranbarger Timothy J, Morcillo Fabienne
DIADE, University Montpellier, CIRAD, IRD, Montpellier, France.
IBMM UMR 5247, CNRS, ENSCM, Université de Montpellier, Montpellier, France.
Methods Mol Biol. 2025;2916:25-38. doi: 10.1007/978-1-0716-4470-6_3.
Jasmonates are a group of plant signaling compounds that control defense mechanisms and modulate growth and development in response to environmental challenges. The protocol in this chapter describes a simple and rapid quantification of the most commonly studied jasmonate, jasmonic acid (JA), and also both its precursor 12-oxo-phytodienoic acid (OPDA) and JA-isoleucine (JA-Ile) the bioactive form, in the abscission zone (AZ) and the mesocarp tissue of oil palm fruits. These tissues are lipid-rich and difficult to analyze because of high levels of triglycerides (TAGs). When TAGs are extracted by apolar solvents, the column and analytical system can be clogged, and the jasmonate quantification is perturbed. In order to determine jasmonate distribution in lipid-rich tissues, we present a protocol to eliminate TAGs to successfully extract and quantify jasmonates, including 12-oxo-phytodienoic acid (OPDA), the most apolar compound, and JA-Ile, the form that binds to the JA co-receptor complex and regulates responses through JA signaling. The protocol is used with the objective of understanding the function of jasmonates in the AZ of ripe oil palm fruit during abscission.
茉莉酸酯是一类植物信号化合物，可控制防御机制，并在应对环境挑战时调节生长和发育。本章中的方案描述了一种简单快速的方法，用于定量分析油棕果实脱落区（AZ）和中果皮组织中最常研究的茉莉酸酯——茉莉酸（JA），以及其前体12-氧代-植物二烯酸（OPDA）和生物活性形式的茉莉酸异亮氨酸（JA-Ile）。这些组织富含脂质，由于甘油三酯（TAGs）含量高而难以分析。当用非极性溶剂提取TAGs时，色谱柱和分析系统可能会堵塞，从而干扰茉莉酸酯的定量分析。为了确定茉莉酸酯在富含脂质组织中的分布，我们提出了一种去除TAGs的方案，以成功提取和定量茉莉酸酯，包括最具非极性的化合物12-氧代-植物二烯酸（OPDA）以及与JA共受体复合物结合并通过JA信号传导调节反应的形式JA-Ile。该方案旨在了解茉莉酸酯在成熟油棕果实脱落过程中脱落区的功能。
