Wu Hao, He Qi, Wang Quan
Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China.
Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, School of Life Sciences, Southwest University, Chongqing, China.
Methods Mol Biol. 2025;2916:99-107. doi: 10.1007/978-1-0716-4470-6_10.
This chapter outlines detailed methods and protocols for studying the structure and mechanisms of abscission layers (ALs) in rice. Utilizing rice spikelets as a primary example, these protocols provide comprehensive techniques for analyzing AL development, including tissue preparation, microscopy, and histochemical assays. The AL is a specialized tissue where cell separation takes place, and it is crucial for processes such as organ shedding and fruit drop. By examining the AL, researchers can uncover the physiological and genetic factors governing plant organ separation. These insights are pertinent for advancing agricultural practices and crop improvement, as understanding the dynamics of the AL can lead to the development of rice varieties with enhanced traits related to abscission and grain retention. These improvements can result in better yield stability and reduced post-harvest losses, which are essential for meeting the food demands of a growing global population. By focusing on the genetic and physiological mechanisms governing the AL, researchers can develop innovative strategies to optimize rice production and contribute to food security.
本章概述了研究水稻脱落层（ALs）结构和机制的详细方法与方案。以水稻小穗作为主要实例，这些方案提供了用于分析脱落层发育的全面技术，包括组织制备、显微镜观察和组织化学分析。脱落层是发生细胞分离的特殊组织，对于器官脱落和落果等过程至关重要。通过研究脱落层，研究人员可以揭示控制植物器官分离的生理和遗传因素。这些见解对于推进农业实践和作物改良具有重要意义，因为了解脱落层的动态变化可以培育出具有与脱落和籽粒保留相关优良性状的水稻品种。这些改进可以提高产量稳定性并减少收获后损失，这对于满足全球不断增长的人口的粮食需求至关重要。通过关注控制脱落层的遗传和生理机制，研究人员可以制定创新策略来优化水稻生产并促进粮食安全。
