Topuz Ruhan Deniz, Cevık Buse, Guler Kursat, Gunduz Ozgur, Karadag Cetin Hakan, Ulugol Ahmet
Faculty of Medicine, Department of Medical Pharmacology, Trakya University, Edirne, Turkey.
World J Biol Psychiatry. 2025 Jun;26(5):199-210. doi: 10.1080/15622975.2025.2502394. Epub 2025 May 14.
Although the brain is not the only source of circulating endocannabinoids and their levels can be affected by many factors, it is underlined that serum endocannabinoid levels can be used as a biomarker in psychiatric disorders. In this study, we aimed to examine whether serum endocannabinoid and N-acylethanolamine concentrations reflect their brain levels.
In the present study acute immobilisation (AIS) and post-traumatic stress (PTSD) models were applied to Wistar albino male rats. Rota rod performance, forced swim, open field and elevated plus maze tests were performed. Endocannabinoid and N-acylethanolamine levels in serum and hippocampus, amygdala and cortex were assessed using LC-MS/MS.
We observed significant increases in anandamide (AEA), palmitoylethanolamide (PEA) and oleoethylethanolamide (OEA) levels in the amygdala and hippocampus in both models except PEA in amygdala in the AIS group, while 2-AG levels decreased. There was no change in serum AEA and 2-AG levels in all groups; in the PTSD group serum PEA levels were higher whereas OEA levels were lower in both the AIS and the PTSD groups.
Our results show that there is no correlation in endocannabinoid and N-acylethanolamine levels between serum and specific brain regions in two stress models of rat.
尽管大脑并非循环内源性大麻素的唯一来源，其水平会受到多种因素影响，但血清内源性大麻素水平可作为精神疾病的生物标志物这一点已得到强调。在本研究中，我们旨在检测血清内源性大麻素和N-酰基乙醇胺浓度是否反映其大脑水平。
在本研究中，将急性制动（AIS）和创伤后应激（PTSD）模型应用于雄性Wistar白化大鼠。进行了转棒试验、强迫游泳试验、旷场试验和高架十字迷宫试验。使用液相色谱-串联质谱法（LC-MS/MS）评估血清、海马体、杏仁核和皮质中的内源性大麻素和N-酰基乙醇胺水平。
我们观察到，在两个模型中，杏仁核和海马体中的花生四烯酸乙醇胺（AEA）、棕榈酰乙醇胺（PEA）和油酰乙醇胺（OEA）水平均显著升高，但AIS组杏仁核中的PEA除外，而2-花生四烯酸甘油（2-AG）水平降低。所有组的血清AEA和2-AG水平均无变化；在PTSD组中，血清PEA水平较高，而在AIS组和PTSD组中，OEA水平均较低。
我们的结果表明，在大鼠的两种应激模型中，血清与特定脑区的内源性大麻素和N-酰基乙醇胺水平之间不存在相关性。
