Dujovny M, Nossovsky N, Diaz F G, Ausman J I, Berman K S
Acta Neurochir (Wien). 1985;77(1-2):62-7. doi: 10.1007/BF01402308.
Lesions generated by the passage of micro-needles through vessel walls are of concern because any lesion may significantly alter hemodynamics of an anastomosis. To study this problem, three different needles were tested on the exposed carotid arteries of 30 rats: the 100 mu taper point, the 100 mu cutting point, and the 75 mu taper point. Trauma generated by the penetration of needles was tested first as the needle and its attached suture was passed through the vessel, then the suture was left in place. One hour after penetration, the arteries were prepared for scanning electron microscopy. Differences between the 100 mu taper point and the 75 mu taper point were significant in terms of size and extent of lesions. An arc of endothelial cells surrounding the wounds showed flattening, tissue destruction and clotting. To minimize endothelial trauma, taper point needles are superior to cutting needles. The 100 mu cutting needle caused damage to the vessel wall in tissue cutting on needle penetration, causing a slit-like incision, and in aggregation of platelets at the puncture site.
微针穿过血管壁所产生的损伤令人担忧，因为任何损伤都可能显著改变吻合处的血流动力学。为研究此问题，在30只大鼠暴露的颈动脉上测试了三种不同的针：100微米锥形尖端针、100微米切割尖端针和75微米锥形尖端针。首先，当针及其附着的缝线穿过血管时，测试针穿透所产生的创伤，然后将缝线留在原位。穿透一小时后，准备对动脉进行扫描电子显微镜检查。100微米锥形尖端针和75微米锥形尖端针在损伤的大小和范围方面存在显著差异。伤口周围的内皮细胞弧显示出扁平、组织破坏和凝血。为使内皮创伤最小化，锥形尖端针优于切割针。100微米切割针在针穿透时的组织切割过程中对血管壁造成损伤，形成狭缝状切口，并在穿刺部位导致血小板聚集。
