Wang Hongjiao, Li Long, Wu Jinhai, Yuan Xuefeng, Hong Liang, Pu Lei, Qin Shunyi, Li Liuan, Yang Hua, Zhang Jianbin
Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China.
College of Food Science, Shanxi Normal University, Shanxi 030606, China.
Poult Sci. 2025 May 7;104(8):105275. doi: 10.1016/j.psj.2025.105275.
Fledgling broiler ducks vary in body weight and growth rate. The aim of this study was to investigate the relationship between serum metabolites and the intestinal microbiota in Cherry Valley broiler ducks with different finishing weights and to reveal differences in their metabolic regulation and microbial composition. Serum and cecum content samples were collected from Cherry Valley broiler ducks of different finishing weights. Metabolites were identified and compared using untargeted metabolomics, 16S rRNA gene sequencing, multivariate statistics and bioinformatics. Six key findings emerged. First, serum biochemical parameters showed that AST and ALT levels were significantly lower in the high weight group (Group H) than in the low weight group (Group L), and serum immunoglobulin IgG levels were significantly higher in group H. Second, the chorionic height to crypt depth ratio of the duodenum was significantly higher in group H than in group L. Third, the gut microbial community diversity or abundance was lower in broiler ducks in group L. Fourth, LEfSe analysis showed that the biomarker for group L was Streptococcus, whereas for group H it was Faecalibacterium. Fifth, a total of 127 differential metabolites were identified (49 up-regulated and 78 down-regulated). Finally, Spearman's correlation analysis showed that Spearman's correlation analyses showed that the Lipid-related serum metabolites were higher in low-body recombinant broiler ducks, mainly Lathosterol, Cholesterol, Cynaratriol and Leukotriene B4. In addition to lipid-associated serum metabolites in high-body recombination, The water-soluble vitamin-like metabolite Pantothenate and the antibiotic-like metabolite Tylosin were high. The cecum microbiota is strongly associated with metabolites, especially Faecalibacterium, unclassified Tannerellaceae, Subdoligranulum, Alistipes, and [Ruminococcus] torques_group, with which it exhibits strong Correlation. Broiler ducks with higher body weights have a better intestinal villous structure, enhanced digestion and absorption, higher levels of immunoglobulin secretion and superior growth performance. Broiler ducks with different body weights differed in plasma metabolites and cecum flora. Spearman's correlation analyses showed that the Correlation between differential metabolites and differential gut microbial genera.
雏肉鸭的体重和生长速度各不相同。本研究的目的是调查不同出栏体重的樱桃谷肉鸭血清代谢物与肠道微生物群之间的关系，并揭示它们在代谢调节和微生物组成上的差异。从不同出栏体重的樱桃谷肉鸭中采集血清和盲肠内容物样本。使用非靶向代谢组学、16S rRNA基因测序、多元统计和生物信息学对代谢物进行鉴定和比较。得出了六个关键发现。第一，血清生化参数显示，高体重组（H组）的谷草转氨酶（AST）和谷丙转氨酶（ALT）水平显著低于低体重组（L组），且H组血清免疫球蛋白IgG水平显著更高。第二，H组十二指肠绒毛高度与隐窝深度之比显著高于L组。第三，L组肉鸭的肠道微生物群落多样性或丰度较低。第四，线性判别分析效应大小（LEfSe）分析表明，L组的生物标志物是链球菌，而H组是粪杆菌。第五，共鉴定出127种差异代谢物（49种上调和78种下调）。最后，斯皮尔曼相关性分析表明，低体重重组肉鸭中与脂质相关的血清代谢物较高，主要是羊毛甾醇、胆固醇、异壬酸和白三烯B4。除了高体重重组中与脂质相关的血清代谢物外，水溶性维生素样代谢物泛酸和抗生素样代谢物泰乐菌素含量较高。盲肠微生物群与代谢物密切相关，尤其是粪杆菌、未分类的坦纳菌科、亚多格兰菌属、阿里斯杆菌属和扭链瘤胃球菌属菌群，与之呈现出强相关性。体重较高的肉鸭具有更好的肠绒毛结构、更强的消化吸收能力、更高水平的免疫球蛋白分泌和更优的生长性能。不同体重的肉鸭在血浆代谢物和盲肠菌群方面存在差异。斯皮尔曼相关性分析表明了差异代谢物与差异肠道微生物属之间的相关性。
