The IbDof2.1-IbABF2 module regulates abscisic acid responses and proline biosynthesis to enhance drought tolerance in sweetpotato.

Drought is a major abiotic stress that impairs plant growth and development. Developing drought-tolerant crop varieties is an important goal of breeders. Transcription factors belonging to the DNA-binding with one zinc finger (Dof) family regulate plant stress responses and development. However, the roles and regulatory mechanisms of Dof2.1 members in plant stress tolerance are still unclear. Here, we cloned the IbDof2.1 gene from sweetpotato and found that its overexpression significantly enhanced drought tolerance of sweetpotato, whereas IbDof2.1-RNA interference (RNAi) plants displayed the opposite phenotype. The IbDof2.1-overexpression plants showed increased abscisic acid (ABA) and proline contents and stomatal sensitivity to ABA and decreased HO accumulation. Furthermore, we found that IbDof2.1 interacted with ABA-binding factor 2 (IbABF2) and promoted the expression of the proline biosynthesis gene IbP5CS1 to increase proline content, further activating the reactive oxygen species (ROS) scavenging system. These results suggest that the IbDof2.1-IbABF2 module induces stomatal closure and activates the ROS scavenging system by regulating ABA responses and proline biosynthesis to enhance drought tolerance in sweetpotato. Our findings provide novel insights into the roles and regulatory mechanisms of Dof2.1 in plants.