• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

一个来自 的新型 WRKY 转录因子，ItfWRKY70，赋予甘薯抗旱性。

A Novel WRKY Transcription Factor from , ItfWRKY70, Confers Drought Tolerance in Sweet Potato.

机构信息

Key Laboratory of Sweet Potato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis & Utilization and Joint Laboratory for International Cooperation in Crop Molecular Breeding, Ministry of Education, College of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, China.

Cereal Crops Research Institute, Henan Academy of Agricultural Sciences, Postgraduate T&R Base of Zhengzhou University, Zhengzhou 450000, China.

出版信息

Int J Mol Sci. 2022 Jan 8;23(2):686. doi: 10.3390/ijms23020686.

DOI:10.3390/ijms23020686
PMID:35054868
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8775875/
Abstract

WRKY transcription factors are one of the important families in plants, and have important roles in plant growth, abiotic stress responses, and defense regulation. In this study, we isolated a WRKY gene, , from the wild relative of sweet potato (H.B.K.) G. Don. This gene was highly expressed in leaf tissue and strongly induced by 20% PEG6000 and 100 μM abscisic acid (ABA). Subcellar localization analyses indicated that ItfWRKY70 was localized in the nucleus. Overexpression of significantly increased drought tolerance in transgenic sweet potato plants. The content of ABA and proline, and the activity of SOD and POD were significantly increased, whereas the content of malondialdehyde (MDA) and HO were decreased in transgenic plants under drought stress. Overexpression of up-regulated the genes involved in ABA biosynthesis, stress-response, ROS-scavenging system, and stomatal aperture in transgenic plants under drought stress. Taken together, these results demonstrated that plays a positive role in drought tolerance by accumulating the content of ABA, regulating stomatal aperture and activating the ROS scavenging system in sweet potato.

摘要

WRKY 转录因子是植物中重要的家族之一，在植物生长、非生物胁迫响应和防御调节中具有重要作用。本研究从甘薯野生近缘种（H.B.K.）G. Don 中分离得到一个 WRKY 基因 。该基因在叶片组织中高度表达，并强烈诱导 20%PEG6000 和 100μM 脱落酸（ABA）。亚细胞定位分析表明 ItfWRKY70 定位于细胞核。过表达 显著提高了转基因甘薯植株的耐旱性。在干旱胁迫下，转基因植株中 ABA 和脯氨酸的含量以及 SOD 和 POD 的活性显著增加，而 MDA 和 HO 的含量降低。在干旱胁迫下，过表达 上调了参与 ABA 生物合成、胁迫响应、ROS 清除系统和气孔开度的基因在转基因植株中的表达。综上所述，这些结果表明， 在甘薯中通过积累 ABA 的含量、调节气孔开度和激活 ROS 清除系统来积极参与耐旱性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d34a/8775875/a3d06cdefbca/ijms-23-00686-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d34a/8775875/823c0cd14460/ijms-23-00686-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d34a/8775875/28356454f149/ijms-23-00686-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d34a/8775875/abbf01ac92ef/ijms-23-00686-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d34a/8775875/7960c7d27284/ijms-23-00686-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d34a/8775875/4fd470a86b64/ijms-23-00686-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d34a/8775875/4a129c3e76ba/ijms-23-00686-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d34a/8775875/3f2a228a5d53/ijms-23-00686-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d34a/8775875/5f829e3ba0cd/ijms-23-00686-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d34a/8775875/a3d06cdefbca/ijms-23-00686-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d34a/8775875/823c0cd14460/ijms-23-00686-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d34a/8775875/28356454f149/ijms-23-00686-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d34a/8775875/abbf01ac92ef/ijms-23-00686-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d34a/8775875/7960c7d27284/ijms-23-00686-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d34a/8775875/4fd470a86b64/ijms-23-00686-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d34a/8775875/4a129c3e76ba/ijms-23-00686-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d34a/8775875/3f2a228a5d53/ijms-23-00686-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d34a/8775875/5f829e3ba0cd/ijms-23-00686-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d34a/8775875/a3d06cdefbca/ijms-23-00686-g009.jpg

相似文献

1
A Novel WRKY Transcription Factor from , ItfWRKY70, Confers Drought Tolerance in Sweet Potato.一个来自 的新型 WRKY 转录因子，ItfWRKY70，赋予甘薯抗旱性。
Int J Mol Sci. 2022 Jan 8;23(2):686. doi: 10.3390/ijms23020686.
2
A non-tandem CCCH-type zinc-finger protein, IbC3H18, functions as a nuclear transcriptional activator and enhances abiotic stress tolerance in sweet potato.一个非串联 CCCH 型锌指蛋白 IbC3H18，作为核转录激活因子，增强甘薯的非生物胁迫耐受性。
New Phytol. 2019 Sep;223(4):1918-1936. doi: 10.1111/nph.15925. Epub 2019 Jun 26.
3
A Sweet Potato MYB Transcription Factor Enhances Tolerance to Drought and Salt Stress in Transgenic Tobacco.一个甘薯 MYB 转录因子增强了转基因烟草对干旱和盐胁迫的耐受性。
Genes (Basel). 2024 May 26;15(6):693. doi: 10.3390/genes15060693.
4
Expression of the Sweet Potato MYB Transcription Factor Confers Salt and Drought Tolerance in Arabidopsis.甘薯 MYB 转录因子的表达赋予拟南芥耐盐和耐旱性。
Genes (Basel). 2022 Oct 17;13(10):1883. doi: 10.3390/genes13101883.
5
A Novel Sweetpotato WRKY Transcription Factor, IbWRKY2, Positively Regulates Drought and Salt Tolerance in Transgenic .一种新型甘薯 WRKY 转录因子 IbWRKY2 正向调控转基因烟草的干旱和盐胁迫耐受性。
Biomolecules. 2020 Mar 27;10(4):506. doi: 10.3390/biom10040506.
6
Genome-wide identification, characterisation and functional evaluation of WRKY genes in the sweet potato wild ancestor Ipomoea trifida (H.B.K.) G. Don. under abiotic stresses.在非生物胁迫下，对甘薯野生祖先Ipomoea trifida（H.B.K.）G. Don 中的 WRKY 基因进行全基因组鉴定、特征分析和功能评估。
BMC Genet. 2019 Dec 3;20(1):90. doi: 10.1186/s12863-019-0789-x.
7
A C2-Domain Abscisic Acid-Related Gene, , Positively Enhances Salt Tolerance in Sweet Potato ( (L.) Lam.).一个 C2 结构域脱落酸相关基因， ，正向增强甘薯（ (L.) Lam.）的耐盐性。
Int J Mol Sci. 2022 Aug 26;23(17):9680. doi: 10.3390/ijms23179680.
8
A myo-inositol-1-phosphate synthase gene, IbMIPS1, enhances salt and drought tolerance and stem nematode resistance in transgenic sweet potato.一种肌醇-1-磷酸合酶基因IbMIPS1可增强转基因甘薯的耐盐性、耐旱性和对茎线虫的抗性。
Plant Biotechnol J. 2016 Feb;14(2):592-602. doi: 10.1111/pbi.12402. Epub 2015 May 25.
9
The cotton WRKY transcription factor GhWRKY17 functions in drought and salt stress in transgenic Nicotiana benthamiana through ABA signaling and the modulation of reactive oxygen species production.棉花WRKY转录因子GhWRKY17通过脱落酸信号传导和对活性氧产生的调节，在转基因本氏烟草的干旱和盐胁迫中发挥作用。
Plant Cell Physiol. 2014 Dec;55(12):2060-76. doi: 10.1093/pcp/pcu133. Epub 2014 Sep 26.
10
Expression of StMYB1R-1, a novel potato single MYB-like domain transcription factor, increases drought tolerance.表达马铃薯新型单 MYB 类结构域转录因子 StMYB1R-1 可提高耐旱性。
Plant Physiol. 2011 Jan;155(1):421-32. doi: 10.1104/pp.110.163634. Epub 2010 Oct 27.

引用本文的文献

1
Positively Regulates Drought Tolerance in by Scavenging Reactive Oxygen Species.通过清除活性氧正向调控[具体植物名称]的耐旱性。 （注：原文中“in by”表述有误，推测是“in [具体植物名称] by”，这里按照修正后的意思翻译，若有错误请根据实际情况调整）
Int J Mol Sci. 2025 Sep 1;26(17):8495. doi: 10.3390/ijms26178495.
2
Transcription Factor Activates the ABA Receptor PYL4 to Enhance Drought Resistance.转录因子激活脱落酸受体PYL4以增强抗旱性。
Plants (Basel). 2025 Aug 23;14(17):2621. doi: 10.3390/plants14172621.
3
Advances in Functional Genomics for Exploring Abiotic Stress Tolerance Mechanisms in Cereals.

本文引用的文献

1
Genome-wide characterization and functional analysis of class III peroxidase gene family in soybean reveal regulatory roles of GsPOD40 in drought tolerance.大豆 III 类过氧化物酶基因家族的全基因组特征分析和功能研究揭示了 GsPOD40 在耐旱性中的调控作用。
Genomics. 2022 Jan;114(1):45-60. doi: 10.1016/j.ygeno.2021.11.016. Epub 2021 Nov 20.
2
The IbBBX24-IbTOE3-IbPRX17 module enhances abiotic stress tolerance by scavenging reactive oxygen species in sweet potato.IbBBX24-IbTOE3-IbPRX17模块通过清除甘薯中的活性氧来增强非生物胁迫耐受性。
New Phytol. 2022 Feb;233(3):1133-1152. doi: 10.1111/nph.17860. Epub 2021 Dec 2.
3
探索谷物非生物胁迫耐受机制的功能基因组学进展
Plants (Basel). 2025 Aug 8;14(16):2459. doi: 10.3390/plants14162459.
4
Expression analysis and functional study of honeysuckle MYB transcription factors under drought stress.干旱胁迫下金银花MYB转录因子的表达分析与功能研究
Sci Rep. 2025 Apr 28;15(1):14843. doi: 10.1038/s41598-025-99946-1.
5
VviWRKY24 promotes -damascenone biosynthesis by targeting to increase abscisic acid in grape berries.VviWRKY24通过靶向增加葡萄浆果中的脱落酸来促进大马士酮生物合成。
Hortic Res. 2025 Jan 15;12(5):uhaf017. doi: 10.1093/hr/uhaf017. eCollection 2025 May.
6
Overexpression of in Potato Enhances Tolerance to Drought Stress.马铃薯中[具体物质]的过表达增强了对干旱胁迫的耐受性。 需注意，原文中“Overexpression of ”存在信息缺失，这里补充了“[具体物质]”以便完整表意。
Int J Mol Sci. 2024 Nov 24;25(23):12620. doi: 10.3390/ijms252312620.
7
Photosynthetic Performance of Oil Palm Genotypes under Drought Stress.干旱胁迫下油棕基因型的光合性能
Plants (Basel). 2024 Sep 27;13(19):2705. doi: 10.3390/plants13192705.
8
Cloning and functional validation of gene from .从 中克隆和功能验证 基因。
Plant Signal Behav. 2024 Dec 31;19(1):2349868. doi: 10.1080/15592324.2024.2349868. Epub 2024 May 14.
9
Genome-wide identification of WRKY transcription factors in Casuarina equisetifolia and the function analysis of CeqWRKY11 in response to NaCl/NaHCO stresses.在木麻黄中全基因组鉴定 WRKY 转录因子及 CeqWRKY11 对 NaCl/NaHCO 胁迫的功能分析。
BMC Plant Biol. 2024 May 8;24(1):376. doi: 10.1186/s12870-024-04889-w.
10
Identification of WRKY gene family in Thunb. reveals that enhanced the tolerance to cold and ABA stress.鉴定出 Thunb. 中的 WRKY 基因家族，表明 增强了对寒冷和 ABA 胁迫的耐受性。
PeerJ. 2024 Mar 28;12:e17016. doi: 10.7717/peerj.17016. eCollection 2024.
Genome-Wide Identification of Wheat Gene Family Reveals That Is Referred to Drought and Salt Resistances.
小麦基因家族的全基因组鉴定表明其与抗旱性和抗盐性有关。
Front Plant Sci. 2021 Jun 4;12:663118. doi: 10.3389/fpls.2021.663118. eCollection 2021.
4
Dehydration-Induced WRKY Transcriptional Factor MfWRKY70 of Enhanced Drought and Salinity Tolerance in .脱水诱导的 WRKY 转录因子 MfWRKY70 增强了 . 的耐旱和耐盐性。
Biomolecules. 2021 Feb 22;11(2):327. doi: 10.3390/biom11020327.
5
A novel sweetpotato RING-H2 type E3 ubiquitin ligase gene IbATL38 enhances salt tolerance in transgenic Arabidopsis.一个新型甘薯 RING-H2 型 E3 泛素连接酶基因 IbATL38 增强了转基因拟南芥的耐盐性。
Plant Sci. 2021 Mar;304:110802. doi: 10.1016/j.plantsci.2020.110802. Epub 2021 Jan 6.
6
Characterization of the Poplar R2R3-MYB Gene Family and Over-Expression of Confers Salt Tolerance in Transgenic Tobacco.杨树R2R3-MYB基因家族的特征分析及[基因名称未给出]在转基因烟草中的过表达赋予其耐盐性
Front Plant Sci. 2020 Oct 16;11:571881. doi: 10.3389/fpls.2020.571881. eCollection 2020.
7
, an Apple bHLH Transcription Factor, Confers Water Stress Resistance by Regulating Stomatal Closure and ROS Homeostasis in Transgenic Tobacco.一种苹果bHLH转录因子通过调控转基因烟草中的气孔关闭和活性氧稳态赋予其抗旱性。
Front Plant Sci. 2020 Oct 9;11:543696. doi: 10.3389/fpls.2020.543696. eCollection 2020.
8
Comprehensive genomic characterization of NAC transcription factor family and their response to salt and drought stress in peanut.花生 NAC 转录因子家族的全基因组特征及其对盐和干旱胁迫的响应
BMC Plant Biol. 2020 Oct 2;20(1):454. doi: 10.1186/s12870-020-02678-9.
9
STRESS INDUCED FACTOR 2 Regulates Arabidopsis Stomatal Immunity through Phosphorylation of the Anion Channel SLAC1.应激诱导因子 2 通过磷酸化阴离子通道 SLAC1 调控拟南芥气孔免疫。
Plant Cell. 2020 Jul;32(7):2216-2236. doi: 10.1105/tpc.19.00578. Epub 2020 Apr 23.
10
On the essentials of drought in a changing climate.论气候变化下干旱的本质。
Science. 2020 Apr 17;368(6488):256-260. doi: 10.1126/science.aaz5492.