优化甲状腺素水平以增强水牛精子冷冻保存及生育能力：聚焦质量、活力和抗氧化保护

Optimizing thyroxine levels for enhanced buffalo sperm cryopreservation and fertility: a focus on quality, viability, and antioxidant protection.

作者信息

Salama Maha S, Ashour Mohey A, Shehabeldin Ahmed M, Omar Mohamed E A, Soliman Mohamed M, Ghamry Heba I, Abdelmegeid Mohamed, Shukry Mustafa, Elolimy Ahmed A

机构信息

Animal Reproduction Research Institute (ARRI), Agricultural Research Center (ARC), Giza, Egypt.

Animal Production Research Institute (APRI), Agricultural Research Center (ARC), Dokki, Egypt.

出版信息

Front Vet Sci. 2025 Apr 30;12:1584903. doi: 10.3389/fvets.2025.1584903. eCollection 2025.

DOI:10.3389/fvets.2025.1584903

PMID:40370824

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12075423/

Abstract

INTRODUCTION

This study investigated the effects of adding thyroxine (T4) to buffalo semen on sperm quality, oxidative markers, apoptosis-like changes, and fertility.

METHODS

Initially, we tested a wide range of T4 concentrations (0.1, 0.3, 0.9, 2.7, and 8.1 µg/dL) to evaluate their impact on motility and viability.

RESULT AND DISCUSSION

Lower concentrations (0.1-0.9 µg/dL) improved total and progressive motility and viability compared to higher concentrations (2.7 and 8.1 µg/dL). We assessed optimized doses (0.25, 0.5, 0.75, and 1 µg/dL) based on these findings. The 0.75 µg/dL group showed superior sperm velocity, viability, motion parameters, membrane, acrosome, and DNA integrity in equilibrated and frozen-thawed samples. Antioxidant markers (GPx, SOD, TAC) were enhanced, while MDA and apoptotic/necrotic cell levels were reduced, particularly in the 0.75 µg/dL group. Fertility trials revealed higher cryosurvival and conception rates in thyroxine-treated groups. In conclusion, T4 supplementation, especially at 0.75 µg/dL, enhances cryopreservation outcomes and fertility potential of buffalo bull semen.

摘要

引言

本研究调查了向水牛精液中添加甲状腺素（T4）对精子质量、氧化标志物、凋亡样变化和生育力的影响。

方法

最初，我们测试了多种T4浓度（0.1、0.3、0.9、2.7和8.1μg/dL），以评估它们对活力和生存力的影响。

结果与讨论

与较高浓度（2.7和8.1μg/dL）相比，较低浓度（0.1 - 0.9μg/dL）可提高总活力、渐进性活力和生存力。基于这些发现，我们评估了优化剂量（0.25、0.5、0.75和1μg/dL）。0.75μg/dL组在平衡和冻融样本中显示出更高的精子速度、生存力、运动参数、膜、顶体和DNA完整性。抗氧化标志物（GPx、SOD、TAC）增强，而MDA以及凋亡/坏死细胞水平降低，尤其是在0.75μg/dL组。生育力试验显示甲状腺素处理组的冷冻存活率和受孕率更高。总之，补充T4，尤其是0.75μg/dL，可提高水牛公牛精液的冷冻保存效果和生育潜力。