Animal Reproduction Research Institute (ARRI), Agricultural Research Center (ARC), Giza, Egypt.
Animal Production Research Institute (APRI), Agricultural Research Center (ARC), Dokki, Egypt.
BMC Vet Res. 2024 Jun 7;20(1):250. doi: 10.1186/s12917-024-04022-x.
Buffalo spermatozoa have a distinct membrane structure that makes them more vulnerable to cryopreservation, resulting in lower-quality post-thawed sperm. This decreases the success rate of artificial insemination in buffaloes. Understanding and addressing these specific vulnerabilities are essential for improving reproductive techniques in buffalo populations. The properties of cryopreserved buffalo bull semen were examined in this study regarding the impact of adding autologous platelet-rich plasma (PRP) to OptiXcell® or Tris egg yolk-based extenders. Ten buffalo bulls were used to collect semen. Each bull's ejaculate was separated into two main equal amounts, each of which was then diluted with either OptiXcell® or Tris egg yolk-based extender, supplemented with various PRP concentrations (5%, 10%, and 15%), and the control (0%), before being cryopreserved according to established protocols. Following equilibration and thawing, the quality and functionality of the sperm were evaluated, along with the antioxidant enzyme activities (GSH and TAC), malondialdehyde (MDA) content, and in vivo fertilization rate of the thawed semen.
All PRP concentrations in both extenders, particularly 10% PRP, improved the quality and functionality of the sperm in both equilibrated and frozen-thawed semen. Additionally, the antioxidant enzyme activities in both extenders were higher in the PRP-supplemented groups compared to the control group in thawed semen (P < 0.05). All post-thaw sperm quality, antioxidant enzyme activities, and functionality aside from DNA integrity were higher (P < 0.05) in the PRP-supplemented OptiXcell® than in the PRP-supplemented Tris egg yolk-based extender. The fertility of cryopreserved semen in the extenders supplemented with 10% and 15% PRP increased (P < 0.05) significantly more than that of the control extenders, with 10% PRP being the optimum concentration in OptiXcell® (80%) compared to that of Tris egg yolk-based extender (66.67%) and control of two extenders (53.33% and 46.67%, respectively).
Even though autologous PRP-supplemented extenders have a protective impact on equilibrated and cryopreserved semen, 10% PRP-supplemented OptiXcell® extenders are more effective at preserving post-thaw semen quality, functionality, and antioxidant capacity, which increases the in vivo fertility of buffalo bulls.
水牛精子具有独特的膜结构，这使得它们在冷冻保存过程中更容易受到损伤，导致解冻后的精子质量下降。这降低了水牛人工授精的成功率。了解和解决这些特定的脆弱性对于提高水牛种群的繁殖技术至关重要。本研究探讨了在 OptiXcell®或 Tris 卵黄基础稀释液中添加自体富血小板血浆（PRP）对冷冻保存水牛公牛精液特性的影响。使用 10 头水牛公牛采集精液。每头公牛的精液分为两个相等的部分，每个部分分别用 OptiXcell®或 Tris 卵黄基础稀释液稀释，添加不同浓度的 PRP（5%、10%和 15%）和对照（0%），然后按照既定方案进行冷冻保存。在平衡和解冻后，评估精子的质量和功能，以及抗氧化酶活性（GSH 和 TAC）、丙二醛（MDA）含量和解冻精液的体内受精率。
两种稀释液中所有 PRP 浓度，特别是 10% PRP，都提高了平衡和冷冻-解冻精液中精子的质量和功能。此外，与对照组相比，PRP 补充组在解冻精液中的抗氧化酶活性更高（P<0.05）。除 DNA 完整性外，所有解冻后精子质量、抗氧化酶活性和功能均较高（P<0.05）PRP 补充 OptiXcell® 比 PRP 补充 Tris 卵黄基础稀释液。在添加 10%和 15% PRP 的稀释液中冷冻保存精液的生育力显著增加（P<0.05），比对照组明显增加，其中 10% PRP 在 OptiXcell®中是最佳浓度（80%），而 Tris 卵黄基础稀释液（66.67%）和两个稀释液的对照（分别为 53.33%和 46.67%）。
尽管自体 PRP 补充剂对平衡和冷冻保存的精液有保护作用，但添加 10% PRP 的 OptiXcell® 稀释液在保存解冻后精子质量、功能和抗氧化能力方面更有效，从而提高了水牛公牛的体内生育力。
