Song Chunyan, Ji Yuke, Wang Yun, Yang Weihua
Shenzhen Eye Hospital, Shenzhen Eye Medical Center, Southern Medical University, Shenzhen, China.
Front Vet Sci. 2025 Apr 30;12:1574519. doi: 10.3389/fvets.2025.1574519. eCollection 2025.
The subretinal injection technique is an important intraocular drug delivery modality that allows access to the subretinal space to directly act on target cells or the administration of medications, markedly improving the therapeutic efficacy of ocular diseases. Subretinal injection in experimental animals is a commonly used manipulation method for investigating vitreoretinal diseases, particularly when gene therapy and cell therapy studies are involved. In this study, we conducted a systematic review on the injection methods, operation sites, post-injection indicators, as well as the progress and significance of subretinal injection in experimental animals, discussed and compared the advantages and disadvantages of the subretinal injection technique, summarized its specific application of subretinal injection in experimental animals, and explored the development and application of this new technology of subretinal injection, hoping to offer insights that may facilitate the further development of this technology.
视网膜下注射技术是一种重要的眼内给药方式，它能够进入视网膜下间隙，直接作用于靶细胞或给药，显著提高眼部疾病的治疗效果。在实验动物中进行视网膜下注射是研究玻璃体视网膜疾病常用的操作方法，尤其是在涉及基因治疗和细胞治疗研究时。在本研究中，我们对实验动物视网膜下注射的方法、操作部位、注射后指标以及进展和意义进行了系统综述，讨论并比较了视网膜下注射技术的优缺点，总结了其在实验动物中的具体应用，并探讨了视网膜下注射这项新技术的发展与应用，希望能提供有助于该技术进一步发展的见解。
