Ljungström María, Nathanson Lubov, Oltra Elisa
Department of Pathology, School of Medicine, Catholic University of Valencia, Valencia, Spain.
Institute for Neuro-Immune Medicine, Dr. Kiran C. Patel College of Osteopathic Medicine, Nova Southeastern University, Fort Lauderdale, FL, USA.
Methods Mol Biol. 2025;2920:39-55. doi: 10.1007/978-1-0716-4498-0_4.
Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a chronic debilitating neuroimmune disease affecting many organs and systems which, in the absence of validated biomarkers, remains diagnosed by clinical criteria. Extracellular vesicles (EV) in blood come from practically all cells in our body and therefore may carry the disease-specific biomarkers needed for the diagnosis of ME. This chapter presents the methodology used on a single pilot study performed to evaluate this possibility to describe a workflow for EV isolation and the analysis of the miRNAs within, which could serve to interrogate additional cohorts of ME/CFS. Among the diverse nature of EV contents miRNAs may constitute a prominent regulatory layer in the development and progress of complex diseases such as ME/CFS, and therefore their study should be further pursued.
肌痛性脑脊髓炎/慢性疲劳综合征（ME/CFS）是一种慢性衰弱性神经免疫疾病，会影响多个器官和系统。在缺乏经过验证的生物标志物的情况下，该疾病仍通过临床标准进行诊断。血液中的细胞外囊泡（EV）几乎来自我们身体的所有细胞，因此可能携带ME诊断所需的疾病特异性生物标志物。本章介绍了在一项单一试点研究中使用的方法，该研究旨在评估这种可能性，以描述EV分离和其中miRNA分析的工作流程，这可能有助于对更多ME/CFS队列进行研究。在EV内容物的多样性质中，miRNA可能在ME/CFS等复杂疾病的发生和发展中构成一个重要的调节层面，因此对其研究应进一步深入。
