一种从黏菌多头绒泡菌中分离细胞质RNA的方法。

A method for isolation of cytoplasmic RNA from a slime mold, Physarum polycephalum.

作者信息

Wiśniewski J, Fronk J, Toczko K

出版信息

Anal Biochem. 1985 Jul;148(1):245-8. doi: 10.1016/0003-2697(85)90652-9.

Abstract

A procedure for fast and simple preparation of cytoplasmic ribonucleic acid from Physarum polycephalum microplasmodia is described. Microplasmodia are homogenized in a high-magnesium-high-ethylene glycol bis(beta-aminoethyl ether) N,N'-tetraacetic acid buffer and nuclei are pelleted. The supernatant is extracted with sodium dodecyl sulfate-phenol-chloroform and crude RNA is precipitated. This is further purified by selective ethanol precipitation from 6 M guanidinum hydrochloride. This RNA preparation is suitable for direct use in hybridization studies.

摘要

本文描述了一种从多头绒泡菌微原质团快速简便制备细胞质核糖核酸的方法。将微原质团在高镁-高乙二醇双（β-氨基乙基醚）N，N'-四乙酸缓冲液中匀浆，使细胞核沉淀。上清液用十二烷基硫酸钠-苯酚-氯仿萃取，粗RNA沉淀。通过从6M盐酸胍中选择性乙醇沉淀进一步纯化。这种RNA制剂适用于直接用于杂交研究。