Keskin Aysenur, Acar Gozde, Aladag Tugce, Onal Ummugulsum, Baltaci Saltuk Bugra, Mogulkoc Rasim, Baltaci Abdulkerim Kasim
Faculty of Medicine, Department of Physiology, Selcuk University, Konya, Turkey.
Faculty of Medicine, Department of Histology, Selcuk University, Konya, Turkey.
Mol Neurobiol. 2025 May 15. doi: 10.1007/s12035-025-05050-w.
Cerebral ischemia-reperfusion (I/R) is a condition that occurs when blood flow is restored after a temporary interruption and may lead to deterioration in brain functions depending on the time passed. One of the changes in functions is neurological score values. This study aimed to determine the effect of brain ischemia reperfusion and 2-week naringin supplementation on changes in neurological score and neurogenesis in ovariectomized female rats. Experimental groups of 36 Wistar-albino-type female rats were created as follows: control group: no anesthesia or surgical procedure was applied. Ovariectomy-sham brain I/R group: After the ovariectomy was performed under general anesthesia, the carotid artery regions were opened and closed, and sham ischemia-reperfusion was performed, followed by a vehicle application for 2 weeks (2 weeks, 1 ml 0.25% carboxymethylcellulose). Ovariectomy-I/R group: After ovariectomy, carotid arteries were isolated under general anesthesia, ligated for 30 min, and reperfused for 2 weeks after ischemia was performed. Ovariectomy-I/R sham treatment group: After ovariectomy, the carotid arteries were isolated under general anesthesia, then ligated and ischemia was performed for 30 min, and then reperfusion and vehicle application were performed for 2 weeks. Ovariectomy-I/R naringin treatment group: After ovariectomy, carotid arteries were isolated under general anesthesia, ligated for 30 min, and ischemia was performed, followed by naringin application with reperfusion for 2 weeks. Neurological scoring values performed on the 1st, 7th, and 14th days after the surgical procedure significantly increased with ischemia-reperfusion. Also, hippocampus and frontal cortex calbindin, alpha/beta-tubulin, and Neu-N levels were reduced considerably by ischemia-reperfusion. However, it was observed that a 2-week naringin application significantly suppressed the increase in neurological scores. The suppression in neurological score values became more evident in the 2nd week. Our results show that the impairment of motor functions and neurogenesis in the frontal cortex and hippocampus in brain ischemia-reperfusion after ovariectomy in female rats was significantly improved by 2 weeks of naringin supplementation.
脑缺血再灌注（I/R）是一种在血流暂时中断后恢复时发生的情况，根据经过的时间，可能会导致脑功能恶化。功能变化之一是神经学评分值。本研究旨在确定脑缺血再灌注和为期2周的柚皮苷补充对去卵巢雌性大鼠神经学评分变化和神经发生的影响。将36只Wistar白化型雌性大鼠分为以下实验组：对照组：未进行麻醉或手术操作。去卵巢假脑I/R组：在全身麻醉下进行去卵巢手术后，打开并闭合颈动脉区域，进行假缺血再灌注，随后给予赋形剂2周（2周，1毫升0.25%羧甲基纤维素）。去卵巢I/R组：去卵巢后，在全身麻醉下分离颈动脉，结扎30分钟，缺血后再灌注2周。去卵巢I/R假治疗组：去卵巢后，在全身麻醉下分离颈动脉，然后结扎并缺血30分钟，然后再灌注并给予赋形剂2周。去卵巢I/R柚皮苷治疗组：去卵巢后，在全身麻醉下分离颈动脉，结扎30分钟并进行缺血，随后在再灌注时给予柚皮苷2周。手术后第1天、第7天和第14天进行的神经学评分值随着缺血再灌注显著增加。此外，海马体和额叶皮质的钙结合蛋白、α/β-微管蛋白和Neu-N水平因缺血再灌注而显著降低。然而，观察到为期2周的柚皮苷应用显著抑制了神经学评分的增加。神经学评分值的抑制在第2周变得更加明显。我们的结果表明，雌性大鼠去卵巢后脑缺血再灌注时额叶皮质和海马体的运动功能损伤和神经发生通过为期2周的柚皮苷补充得到了显著改善。
