Ali Marwa A, Shaker Olfat G, Ezzat Eman M, Khalefa Abeer A, Ahmed Naglaa A, Abu El-Ela Raghda Yahia, Amin Amal A Ibrahim, Mohamed Fatma Abdel Hamid, Abdelhafeez Marwa Mamdouh Ahmed, Ahmed Mohamed Saad Zaghloul, AbdelHafez Marwa N, Abdulqader Abdulrhman Khaled Al, Fares Reham
Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Fayoum University, Fayoum, Egypt.
Department of Biomedical Sciences, College of Medicine, King Faisal University, Al-Ahsa, Saudi Arabia.
J Clin Lab Anal. 2025 Jun;39(11):e70039. doi: 10.1002/jcla.70039. Epub 2025 May 16.
Colorectal cancer (CRC) is a worldwide burden. Circular RNAs are promising biomarkers for diagnosing and prognosis of CRC.
To investigate the possible association of sera levels of CircFUNDC1 and CircUHRF1 expression with predisposition and clinicopathological findings in CRC, ulcerative colitis (UC), and Crohn's disease (CD) in Egyptian patients.
The serum levels of CircFUNDC1 and CircUHRF1 were evaluated in 113 Egyptian subjects divided into four groups; CRC (31), UC (26), and CD (25) and compared to healthy controls (31) using quantitative polymerase chain reaction.
The median values of log2 serum fold change (FC) of CircFUNDC1 in CRC, UC, and CD patients were 9.11, 6.58, and 6.17, respectively. It was upregulated in all case groups. CRC, UC, and CD patients had significantly higher serum CircFUNDC1 levels than controls (p < 0.001). However, there were no significant differences among patient groups (CRC, UC, and CD). The medians of log 2 of serum FC CircUHRF1 in patients with CRC, UC, and CD were -2.00, 3.33, and 3.12, respectively. The CircUHRF1 serum level was lower in the CRC group of patients, with no significant difference between the CRC group and the controls. Serum CircUHRF1 was significantly overexpressed in patients with UC and CD compared to the CRC groups or controls (p < 0.001). By Roc curve analysis, both genes can differentiate CRC patients from inflammatory bowel disease (IBD) patients or healthy controls with p < 0.05.
Serum CircFUNDC1 is a biomarker for CRC, while CircUHRF1 is a biomarker of IBD.
结直肠癌（CRC）是一项全球性负担。环状RNA是用于结直肠癌诊断和预后的有前景的生物标志物。
探讨埃及患者血清中CircFUNDC1和CircUHRF1表达水平与结直肠癌、溃疡性结肠炎（UC）和克罗恩病（CD）的易感性及临床病理结果之间的可能关联。
对113名埃及受试者进行分组，分为四组：结直肠癌组（31例）、溃疡性结肠炎组（26例）、克罗恩病组（25例），并与健康对照组（31例）进行比较，采用定量聚合酶链反应评估血清中CircFUNDC1和CircUHRF1的水平。
结直肠癌、溃疡性结肠炎和克罗恩病患者中CircFUNDC1的log2血清倍数变化（FC）中位数分别为9.11、6.58和6.17。在所有病例组中均上调。结直肠癌、溃疡性结肠炎和克罗恩病患者的血清CircFUNDC1水平显著高于对照组（p < 0.001）。然而，患者组（结直肠癌、溃疡性结肠炎和克罗恩病）之间无显著差异。结直肠癌、溃疡性结肠炎和克罗恩病患者血清FC CircUHRF1的log2中位数分别为-2.00、3.33和3.12。结直肠癌组患者的CircUHRF1血清水平较低，结直肠癌组与对照组之间无显著差异。与结直肠癌组或对照组相比，溃疡性结肠炎和克罗恩病患者的血清CircUHRF1显著过表达（p < 0.001）。通过Roc曲线分析，两个基因均可将结直肠癌患者与炎症性肠病（IBD）患者或健康对照组区分开来，p < 0.05。
血清CircFUNDC1是结直肠癌的生物标志物，而CircUHRF1是炎症性肠病的生物标志物。
