Kirchgessner M, Reithmayer F, Roth-Maier D A
Ann Nutr Metab. 1985;29(3):138-46. doi: 10.1159/000176957.
In order to study the influence of varying vitamin B6 intake levels on the reproductive performance and the vitamin B6 status of fetuses and reproductive organs, 30 pregnant rats (Sprague Dawley strain) were fed a semisynthetic casein diet supplemented with 1, 6, 12, 24 and 48 mg vitamin B6/kg throughout pregnancy. On days 19 of pregnancy the rats were killed. Fetuses, placenta, amniotic fluid, amnion, uterus and mammary gland were analysed microbiologically for their vitamin B6 content. Reproductive performance was not influenced by the dietary treatment. Vitamin B6 concentrations in fetuses, placenta and amniotic fluid of mothers treated with the low 1-ppm vitamin B6 diet were significantly decreased. At a vitamin B6 supply of 6 ppm, however, tissue saturation was already reached in these organs. Accordingly, the whole vitamin B6 retention in the reproductive product plateaued at an intake level of 6 ppm vitamin B6 at 37 micrograms vitamin B6 per animal. These results indicate therefore that a level of 6 ppm dietary vitamin B6 is sufficient to guarantee an optimum vitamin B6 status in the reproductive product, and higher vitamin B6 supplementations showed no further effect on the parameters investigated.
为了研究不同维生素B6摄入水平对繁殖性能以及胎儿和生殖器官的维生素B6状态的影响，在整个孕期给30只怀孕的大鼠（斯普拉格-道利品系）喂食添加了1、6、12、24和48毫克维生素B6/千克的半合成酪蛋白日粮。在怀孕第19天处死大鼠。对胎儿、胎盘、羊水、羊膜、子宫和乳腺进行微生物学分析，测定其维生素B6含量。繁殖性能不受日粮处理的影响。用低至1 ppm维生素B6日粮处理的母鼠所产胎儿、胎盘和羊水中的维生素B6浓度显著降低。然而，在维生素B6供应量为6 ppm时，这些器官已达到组织饱和。因此，生殖产物中维生素B6的总保留量在维生素B6摄入量为6 ppm时达到平稳状态，每只动物为37微克维生素B6。因此，这些结果表明，日粮中6 ppm的维生素B6水平足以保证生殖产物中的维生素B6状态达到最佳，更高的维生素B6补充量对所研究的参数没有进一步影响。
