Mahajan Janhavi, Padula Matthew P, Marks Denese C, Johnson Lacey
Research and Development, Australian Red Cross Lifeblood, Alexandria, New South Wales, Australia.
School of Life Sciences, University of Technology Sydney, Sydney, New South Wales, Australia.
Transfusion. 2025 Jul;65(7):1307-1318. doi: 10.1111/trf.18283. Epub 2025 May 16.
Cold storage (2-6°C) preserves the aggregatory and clot formation properties of platelets beyond the standard 7-day shelf life. Comparatively little is known about the effect of cold storage on clot resolution functions, which are necessary to ensure normal hemostatic balance is maintained.
Double-dose apheresis platelets (n = 8) were collected using the Trima apheresis platform (40% plasma/60% PAS-E). Platelets were sampled on Day 1 post-collection and allocated to room temperature (RT; 20-24°C) or cold storage (2-6°C). In vitro testing was performed over a 21-day period.
The coagulation profile of cold-stored platelets was better maintained throughout storage compared to RT platelets. Cold-stored platelets showed increased thrombin generation potential (faster lag time, higher peak, and increased ETP) compared to RT platelets throughout storage. By viscoelastic testing, the IN-test and EX-test clotting times (CT) were maintained over cold storage for 21 days, while the clot strength (MCF) decreased by approximately 15%. The lysis time (LT) was unchanged during cold storage. Macroscopically, less clot retraction and a higher percentage of internal lysis were observed in cold-stored platelets; however, susceptibility to external lysis was not affected.
Hemostasis requires a delicate balance between clot formation and resolution, and we have shown that the key functional properties of cold platelets stored in PAS-E are well-regulated during extended storage for 21 days.
冷藏（2-6°C）可使血小板的聚集和凝血形成特性在标准的7天保质期后仍得以保留。关于冷藏对凝血溶解功能的影响，人们所知相对较少，而凝血溶解功能对于确保维持正常的止血平衡是必不可少的。
使用Trima血细胞分离平台（40%血浆/60% PAS-E）采集双倍剂量的单采血小板（n = 8）。在采集后第1天对血小板进行采样，并分配至室温（RT；20-24°C）或冷藏（2-6°C）条件下。在21天的时间内进行体外测试。
与室温保存的血小板相比，冷藏保存的血小板在整个保存期间凝血特性得到了更好的维持。在整个保存期间，与室温保存的血小板相比，冷藏保存的血小板显示出更高的凝血酶生成潜力（滞后时间更短、峰值更高以及内源性凝血酶潜能增加）。通过黏弹性测试，IN-测试和EX-测试的凝血时间（CT）在冷藏21天期间得以维持，而凝块强度（MCF）下降了约15%。在冷藏期间，溶解时间（LT）没有变化。宏观上，冷藏保存的血小板观察到凝块回缩较少且内部溶解的百分比更高；然而，对外源溶解的敏感性未受影响。
止血需要在凝血形成和溶解之间保持微妙的平衡，并且我们已经表明，保存在PAS-E中的冷藏血小板的关键功能特性在延长的21天保存期内得到了良好的调节。
