褐飞虱内共生菌砷虫菌的分离、培养及人工感染实验方案
Protocol for isolating, culturing, and artificially infecting the Arsenophonus endosymbiont of Nilaparvata lugens.
作者信息
Cai Tingwei, Wan Hu
机构信息
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China; Hubei Hongshan Laboratory, Wuhan 430070, China; Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China; Hubei Hongshan Laboratory, Wuhan 430070, China; Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
出版信息
STAR Protoc. 2025 Jun 20;6(2):103833. doi: 10.1016/j.xpro.2025.103833. Epub 2025 May 15.
Endosymbionts play a crucial role in insect physiology and adaptation. Here, we present a protocol for isolation, cultivation, and artificial infection of the Arsenophonus endosymbiont of Nilaparvata lugens. We describe steps for symbiont isolation, in vitro culture maintenance, genetic modification, and host reinfection. This protocol enables controlled studies of symbiont-host interactions and provides a foundation for symbiont-based pest management strategies. For complete details on the use and execution of this protocol, please refer to Cai et al..
内共生菌在昆虫生理学和适应性方面发挥着关键作用。在此，我们展示了一种用于分离、培养和人工感染褐飞虱体内的阿氏共生菌（Arsenophonus）的方案。我们描述了共生菌分离、体外培养维持、基因改造以及宿主再感染的步骤。该方案能够对共生菌与宿主的相互作用进行可控研究，并为基于共生菌的害虫管理策略提供基础。有关该方案使用和执行的完整详细信息，请参考蔡等人的研究。
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