Wong Yiu-Tung, Chan Ka-Lok, Yiu Odi Hang-Wah, Chen Chun-Ching, Leung Ting-Wai, Yeung Kin-Wai
Ho Man Tin Government Offices, Analytical and Advisory Services Division, Government Laboratory, 7/F, 88 Chung Hau Street, Ho Man Tin, Kowloon, Hong Kong Special Administrative Region, China.
J AOAC Int. 2025 Jul 1;108(4):558-565. doi: 10.1093/jaoacint/qsaf053.
Lauric arginate ethyl ester (LAE) is a natural cationic surfactant exhibiting excellent antimicrobial activity and has been approved as a safe additive in certain food according to the Codex Alimentarius Commission (CAC) General Standard for Food Additives (GSFA).
This study aimed to develop an analytical method based on liquid chromatography tandem mass spectrometry (LC-MS/MS) for the analysis of LAE in food using isotope-labeled LAE as internal standard.
LAE in food was extracted by a mixture of acetonitrile (ACN)-water (9:1) and analyzed by LC-MS/MS via an internal standard calibration method using LAE-d23 hydrochloride as the internal standard.
The limit of detection values of LAE were 0.32 mg/kg in liquid samples and 1.0 mg/kg in solid samples. The limit of quantitation values were 4.4 mg/kg and 14 mg/kg in liquid and solid samples, respectively. Spike recoveries consistently fell within the range of 90 to 110% at three different fortification levels, accompanied by a RSD below 10% in each instance.
An analytical method with LC-MS/MS detection for the analysis of LAE in various food matrixes was successfully developed, validated, and demonstrated to be fast, robust, and reliable.
The developed analytical method with LC-MS/MS detection offered a fast and efficient way to analyze LAE in various food samples during a routine food surveillance program.
月桂酸精氨酸乙酯（LAE）是一种天然阳离子表面活性剂，具有出色的抗菌活性，并且根据食品法典委员会（CAC）食品添加剂通用标准（GSFA）已被批准作为某些食品中的安全添加剂。
本研究旨在开发一种基于液相色谱串联质谱法（LC-MS/MS）的分析方法，以同位素标记的LAE作为内标来分析食品中的LAE。
食品中的LAE用乙腈（ACN）-水（9:1）混合物提取，并通过LC-MS/MS采用内标校准法进行分析，以盐酸LAE-d23作为内标。
LAE在液体样品中的检测限为0.32mg/kg，在固体样品中的检测限为1.0mg/kg。液体和固体样品中的定量限分别为4.4mg/kg和14mg/kg。在三个不同的加标水平下，加标回收率始终在90%至110%的范围内，且每次的相对标准偏差均低于10%。
成功开发、验证了一种采用LC-MS/MS检测分析各种食品基质中LAE的分析方法，该方法快速、稳健且可靠。
所开发的采用LC-MS/MS检测的分析方法为常规食品监测计划中分析各种食品样品中的LAE提供了一种快速有效的方法。
