兔角膜对局部应用视黄醇和全反式维甲酸的体内代谢

In vivo metabolism of topically applied retinol and all-trans retinoic acid by the rabbit cornea.

作者信息

Ubels J L, Edelhauser H F

出版信息

Biochem Biophys Res Commun. 1985 Aug 30;131(1):320-7. doi: 10.1016/0006-291x(85)91805-4.

Abstract

Corneas of normal and vitamin A-deficient rabbits were treated topically with [11, 12-3H] retinol or [11, 12-3H] all-trans retinoic acid. Methanol extracts of these corneas were analyzed by high pressure liquid chromatography. Radiolabeled compounds were extracted from the corneas which co-migrated chromatographically with known retinoid standards. In agreement with studies on other tissues and organs, retinol was metabolized to retinoic acid and more polar compounds by corneas of normal and vitamin A-deficient rabbits. All-trans retinoic acid was isomerized to 13-cis retinoic acid in normal rabbit corneas; however, this trans-cis isomerization did not occur in vitamin A-deficient, xerophthalmic corneas.

摘要

用[11, 12-³H]视黄醇或[11, 12-³H]全反式维甲酸对正常和维生素A缺乏的兔角膜进行局部处理。通过高压液相色谱法分析这些角膜的甲醇提取物。从角膜中提取出放射性标记化合物，其在色谱上与已知类视黄醇标准品共迁移。与对其他组织和器官的研究一致，正常和维生素A缺乏的兔角膜将视黄醇代谢为维甲酸和更多极性化合物。在正常兔角膜中，全反式维甲酸异构化为13-顺式维甲酸；然而，这种反-顺异构化在维生素A缺乏的干眼角膜中并未发生。