Bomzon A, Blendis L M, Better O S, Yarhi D, Copel Y, Youdim M B
Biochem Pharmacol. 1985 Sep 1;34(17):3049-54. doi: 10.1016/0006-2952(85)90146-7.
The pulmonary metabolism of noradrenaline (NA) was measured in lungs removed from 3 day sham-operated rats and from rats whose bile ducts had been ligated 3 days earlier (BDL). The pulmonary metabolism of NA as measured by a single clearance of the radio-labelled 14C-amine was significantly increased in lungs excised from BDL rats as compared to that measured in the sham-operated rats. The change in metabolism was associated with an alteration in the pulmonary uptake of NA and not with the activities of the enzymes monoamine oxidase types A and B and catechol-O-methyl transferase. Moreover, it was not correlated with rises in the bilirubin or cholesterol concentrations in the serum of the BDL rats and occurred independent of any changes in pulmonary pressure. In a second series of experiments, the evolution of this abnormality over the period of one to six days postoperative was investigated. In the sham-operated rats, there was no significant change in the pulmonary metabolism of NA even by the sixth day. In contrast, there were time-dependent increases from one to six days in these metabolic processes in BDL rats with the highest values being at six days. In contrast, the serum concentrations of bilirubin and cholesterol and activities of the enzymes, alanine transaminase and alkaline phosphatase all rose to their maximum by the fourth day and thereafter declined. Although serum albumin levels fell significantly in BDL rats they were not significantly different from sham-controls. Thus, change in pulmonary metabolism of NA with obstructive jaundice increases with time from one to six days and it not related to the blood chemical changes of biliary obstruction or hepatic synthetic function.
在从3日龄假手术大鼠以及3天前胆管已被结扎（BDL）的大鼠处取出的肺中，测量了去甲肾上腺素（NA）的肺代谢。与假手术大鼠相比，通过放射性标记的14C - 胺的单次清除率测量的BDL大鼠肺中NA的肺代谢显著增加。代谢变化与NA的肺摄取改变有关，而与A型和B型单胺氧化酶以及儿茶酚 - O - 甲基转移酶的活性无关。此外，它与BDL大鼠血清中胆红素或胆固醇浓度的升高无关，并且与肺压力的任何变化无关而发生。在第二系列实验中，研究了术后1至6天期间这种异常情况的演变。在假手术大鼠中，即使到第6天，NA的肺代谢也没有显著变化。相比之下，BDL大鼠中这些代谢过程在1至6天内呈时间依赖性增加，最高值出现在第6天。相比之下，胆红素和胆固醇的血清浓度以及丙氨酸转氨酶和碱性磷酸酶的活性在第4天均升至最高，此后下降。尽管BDL大鼠的血清白蛋白水平显著下降，但与假手术对照组相比并无显著差异。因此，阻塞性黄疸时NA的肺代谢变化在1至6天内随时间增加，并且与胆道梗阻的血液化学变化或肝脏合成功能无关。
