Wu Lunbi, Han Dongxue, Zhang Xiaodong, Zhang Shixu, Wang Yicong, Han Wenxue, Jiang Bowen, Chen Huailei, Jiang Libo
Qiqihar Medical College, No. 333 Bukui North Street, Qiqihar, Heilongjiang, China.
Department of Laboratory Diagnosis, The Second Affiliated Hospital of Qiqihar Medical College, No. 64, Zhonghua West Road, Qiqihar, 161000, Heilongjiang, China.
Eur J Med Res. 2025 May 19;30(1):392. doi: 10.1186/s40001-025-02655-8.
The study analyzed the relationship between serum levels of HMGB1 and biochemical indices related to atherosclerosis, clarifying the diagnostic value of HMGB1 in cases of myeloproliferative neoplasm (MPN) accompanied by atherosclerosis (AS).
The bone marrow samples and serum were obtained from patients with MPN. qPCR was used to detect the level of HMGN1 mRNA in bone marrow mononuclear cells, while ELISA was used to measure the level of HMGB1 in serum. Additionally, relevant biochemical indices of the patients were collected and the correlation between these indices and HMGB1 levels was assessed.
Compared with the control group, MPN patients exhibited significantly elevated levels of HMGB1 in both bone marrow mononuclear cells and serum. The Pearson correlation analysis revealed a negative correlation between serum HMGB1 levels and both HDL-C and ApoA1. The ROC curve analysis demonstrated that serum HMGB1 had an AUC of 0.929 (P < 0.001) for predicting the complication of AS in MPN patients, with a sensitivity of 100.00% and a specificity of 78.05%. Furthermore, logistic regression analysis showed that serum HMGB1 levels were statistically significant in determining the presence of AS in MPN patients (P < 0.05).
Elevated levels of HMGB1 in the bone marrow and serum of MPN patients demonstrate a correlation with biochemical indices related to AS. This finding may serve as a valuable reference for predicting AS complications in MPN patients.
本研究分析了血清高迁移率族蛋白B1（HMGB1）水平与动脉粥样硬化相关生化指标之间的关系，以阐明HMGB1在伴有动脉粥样硬化（AS）的骨髓增殖性肿瘤（MPN）病例中的诊断价值。
采集MPN患者的骨髓样本和血清。采用qPCR检测骨髓单个核细胞中HMGN1 mRNA水平，采用酶联免疫吸附测定（ELISA）法检测血清中HMGB1水平。此外，收集患者的相关生化指标，并评估这些指标与HMGB1水平之间的相关性。
与对照组相比，MPN患者骨髓单个核细胞和血清中的HMGB1水平均显著升高。Pearson相关性分析显示，血清HMGB1水平与高密度脂蛋白胆固醇（HDL-C）和载脂蛋白A1（ApoA1）均呈负相关。受试者工作特征（ROC）曲线分析表明，血清HMGB1预测MPN患者发生AS并发症的曲线下面积（AUC）为0.929（P < 0.001），敏感性为100.00%，特异性为78.05%。此外，逻辑回归分析显示，血清HMGB1水平在确定MPN患者是否存在AS方面具有统计学意义（P < 0.05）。
MPN患者骨髓和血清中HMGB1水平升高与AS相关生化指标存在相关性。这一发现可为预测MPN患者的AS并发症提供有价值的参考。
