Pagh K I, Vergara J A, Adelman M R
Exp Cell Res. 1985 Jan;156(1):287-93. doi: 10.1016/0014-4827(85)90284-8.
A motile, lamellipodium-like structure, the ridge, forms as amoeboflagellate cells of Physarum polycephalum release from a substratum and begin swimming in fluid. Actin microfilaments form a distinct laminar core within the ridge; they are seen as a sparse, disordered meshwork in cytoskeletons prepared by conventional methods using uranyl acetate negative staining [10]. Preservation and visualization of these filaments and their arrangements improved considerably when cytoskeletons were imaged with phosphotungstic acid buffered with ammonium hydroxide (PTA(NH4]. Microfilaments within ridge cytoskeletons were found to form loose bundles and criss-crossing, 'meshwork' arrays several layers deep. Differences could be detected in morphology and detailed arrangement of microfilaments within cytoskeletons prepared in the presence of phalloidin. PTA(NH4) may be useful for studies of cytoskeletal elements and their rearrangements in dynamic, motile regions of cells.
一种能动的、类似片状伪足的结构——嵴，在多头绒泡菌的变形鞭毛细胞从基质释放并开始在液体中游动时形成。肌动蛋白微丝在嵴内形成一个明显的层状核心；在用醋酸双氧铀负染色的传统方法制备的细胞骨架中，它们表现为稀疏、无序的网络[10]。当用氢氧化铵缓冲的磷钨酸（PTA(NH4)）对细胞骨架进行成像时，这些细丝及其排列的保存和可视化有了很大改善。在嵴细胞骨架内发现微丝形成松散的束状以及几层深的交叉“网络”阵列。在鬼笔环肽存在下制备的细胞骨架内，微丝的形态和详细排列存在差异。PTA(NH4)可能有助于研究细胞骨架元件及其在细胞动态、能动区域的重排。
