Park Hye Rang, Heo Su Vin, Kang Beom Kyu, Seo Hyoseob, Lee Eunsoo, Park Jihee, Jang Yun Woo, Seo Jeong Hyun, Park Girim, Kim Jun Hoi, Lee Yeong Hoon, Han Won Young, Seo Myung Chul, Jeung Ji-Ung
Division of Upland Crop Breeding Research, Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration, Miryang, Republic of Korea.
Division of Crop Production Technology Research, Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration, Miryang, Republic of Korea.
Front Plant Sci. 2025 May 2;16:1520999. doi: 10.3389/fpls.2025.1520999. eCollection 2025.
Phytophthora root and stem rot (PRSR), an infection caused by in soybean [ (L.) Merr.], is an important threat to soybean production in South Korea; however, the precise genetic mechanisms related to PRSR resistance remain largely unclear, limiting the development of resistant cultivars. This study aimed to identify candidate resistance () genes related to PRSR resistance in soybeans through a genome-wide association study (GWAS). We evaluated 205 soybean accessions inoculated with isolates GJ3053 or AD3617 using the hypocotyl inoculation method and genotyped them using a 180K Axiom SoyaSNP chip. We identified 19 significant single-nucleotide polymorphisms (SNPs) related to resistance against one or both of the two isolates: GJ3053 resistance was associated with SNPs on chromosomes 2, 3, 9, 10, 14, and 16, whereas AD3617 resistance was related to SNPs on chromosomes 3, 13, and 16. The SNP AX-90410433 (3,628,549 bp) on chromosome 3 was significantly linked to resistance against both isolates, based on the linkage disequilibrium (480 kb) and -log() values (6.62). This region harbors key resistance gene analogs (RGAs), including nucleotide-binding site leucine-rich repeat and serine-threonine protein kinases. Among the 34 identified RGAs in the 2.94.4 Mbp region, G, which encodes a protein with serine kinase activity, emerged as a strong candidate. Haplotype analysis revealed that this gene exhibited genotypic patterns consistent with the resistance phenotypes of the selected accessions. Validation through gene expression and kompetitive allele-specific PCR marker analysis supported the role of G in PRSR resistance. These findings underscore the significance of identifying and utilizing PRSR resistance genes, such as , to enhance pathogen resistance in soybean breeding programs. Our results can inform the development of cultivars with improved resistance to , thus potentially mitigating the effect of pathogenic stress on crop productivity and quality, and contributing to sustainable agriculture.
疫霉根腐茎腐病（PRSR）是由大豆疫霉（Phytophthora sojae）引起的一种侵染性病害，对韩国的大豆生产构成了重大威胁；然而，与PRSR抗性相关的精确遗传机制在很大程度上仍不清楚，这限制了抗性品种的培育。本研究旨在通过全基因组关联研究（GWAS）鉴定大豆中与PRSR抗性相关的候选抗性（R）基因。我们采用下胚轴接种法对接种了大豆疫霉菌株GJ3053或AD3617的205份大豆种质进行了评估，并使用180K Axiom SoyaSNP芯片对它们进行了基因分型。我们鉴定出19个与对两种菌株中的一种或两种的抗性相关的显著单核苷酸多态性（SNP）：对GJ3053的抗性与2号、3号、9号、10号、14号和16号染色体上的SNP相关，而对AD3617的抗性与3号、13号和16号染色体上的SNP相关。基于连锁不平衡（480 kb）和-log（P）值（6.62），3号染色体上的SNP AX-90410433（3,628,549 bp）与对两种菌株的抗性显著相关。该区域包含关键抗性基因类似物（RGA），包括核苷酸结合位点富含亮氨酸重复序列和丝氨酸-苏氨酸蛋白激酶。在2.94.4 Mbp区域鉴定出的34个RGA中，编码具有丝氨酸激酶活性蛋白的基因G成为一个有力的候选基因。单倍型分析表明，该基因表现出与所选种质的抗性表型一致的基因型模式。通过基因表达和竞争性等位基因特异性PCR标记分析进行的验证支持了基因G在PRSR抗性中的作用。这些发现强调了鉴定和利用PRSR抗性基因（如基因G）以增强大豆育种计划中病原菌抗性的重要性。我们的结果可为培育对大豆疫霉抗性增强的品种提供参考，从而有可能减轻病原胁迫对作物生产力和品质的影响，并为可持续农业做出贡献。
