Li Yinping, Sun Suli, Zhong Chao, Wang Xiaoming, Wu Xiaofei, Zhu Zhendong
National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing, 100081, People's Republic of China.
Theor Appl Genet. 2017 Jun;130(6):1223-1233. doi: 10.1007/s00122-017-2883-7. Epub 2017 Mar 4.
The RpsQ Phytophthora resistance locus was finely mapped to a 118-kb region on soybean chromosome 3. A best candidate gene was predicted and three co-segregating gene markers were developed. Phytophthora root rot (PRR), caused by Phytophthora sojae, is a major threat to sustainable soybean production. The use of genetically resistant cultivars is considered the most effective way to control this disease. The Chinese soybean cultivar Qichadou 1 exhibited a broad spectrum resistance, with a distinct resistance phenotype, following inoculation with 36 Chinese P. sojae isolates. Genetic analyses indicated that the disease resistance in Qichadou 1 is controlled by a single dominant gene. This gene locus was designated as RpsQ and mapped to a 118-kb region between BARCSOYSSR_03_0165 and InDel281 on soybean chromosome 3, and co-segregated with Insert11, Insert144 and SNP276. Within this region, there was only one gene Glyma.03g27200 encoding a protein with a typical serine/threonine protein kinase structure, and the expression pattern analysis showed that this gene induced by P. sojae infection, which was suggested as a best candidate gene of RpsQ. Candidate gene specific marker Insert144 was used to distinguish RpsQ from the other known Rps genes on chromosome 3. Identical polymerase chain reaction amplification products were produced for cultivars Qichadou 1 (RpsQ) and Ludou 4 (Rps9). All other cultivars carrying Rps genes on chromosome 3 produced different PCR products, which all lacked a 144-bp fragment present in Qichadou 1 and Ludou 4. The phenotypes of the analyzed cultivars combined with the physical position of the PRR resistance locus, candidate gene analyses, and the candidate gene marker test revealed RpsQ and Rps9 are likely the same gene, and confer resistance to P. sojae.
RpsQ 疫霉抗性位点被精细定位到大豆 3 号染色体上一个 118 kb 的区域。预测了一个最佳候选基因，并开发了三个共分离基因标记。由大豆疫霉引起的疫霉根腐病（PRR）是可持续大豆生产的主要威胁。使用抗基因品种被认为是控制这种病害的最有效方法。中国大豆品种齐茶豆 1 在接种 36 个中国大豆疫霉菌株后表现出广谱抗性，具有明显的抗性表型。遗传分析表明，齐茶豆 1 的抗病性由一个显性单基因控制。该基因位点被命名为 RpsQ，定位到大豆 3 号染色体上 BARCSOYSSR_03_0165 和 InDel281 之间的一个 118 kb 区域，并与 Insert11、Insert144 和 SNP276 共分离。在该区域内，只有一个基因 Glyma.03g27200 编码具有典型丝氨酸/苏氨酸蛋白激酶结构的蛋白质，表达模式分析表明该基因受大豆疫霉感染诱导，被认为是 RpsQ 的最佳候选基因。候选基因特异性标记 Insert144 用于区分 RpsQ 与 3 号染色体上其他已知的 Rps 基因。品种齐茶豆 1（RpsQ）和鲁豆 4（Rps9）产生相同的聚合酶链反应扩增产物。3 号染色体上携带 Rps 基因的所有其他品种产生不同的 PCR 产物，这些产物都缺少齐茶豆 1 和鲁豆 4 中存在的 144 bp 片段。分析品种的表型与疫霉根腐病抗性位点的物理位置、候选基因分析以及候选基因标记测试相结合，表明 RpsQ 和 Rps9 可能是同一个基因，并赋予对大豆疫霉的抗性。
