金银花提取物作为严重急性呼吸综合征冠状病毒2（SARS-CoV-2）感染的潜在抑制剂
Honeysuckle extracts as a potential inhibitor of SARS-CoV-2 infection.
作者信息
Lin Ping-Chang, Lee Ying-Ray, Liu Li-Teh, Chiou Shyh-Shin, Chen Po-Chih, Tsai Ching-Yi, Tsai Jih-Jin
机构信息
Tropical Medicine Center, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
Department of Microbiology and Immunology, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
出版信息
Front Pharmacol. 2025 Apr 16;16:1517585. doi: 10.3389/fphar.2025.1517585. eCollection 2025.
BACKGROUND
In the current era of coronavirus disease 2019 (COVID-19), we were interested in searching for medications other than the currently available antiviral drugs Paxlovid and Molnupiravir that cause minimal side effects and do not harm the human body. Honeysuckle extract (HSE) is a traditional Chinese medicine (TCM) that has been shown to exert antiviral effects in other studies. However, no studies have indicated whether HSE has an inhibitory effect on SARS-CoV-2.
METHODS
We prepared HSEs from dried honeysuckle flowers. We performed a cell viability assay, median tissue culture infection dose (TCID) assay, and qRT‒PCR, and calculated the virus titers using the Reed-Muench method to evaluate the inhibitory effects of aqueous and alcohol HSEs on SARS‒CoV‒2 and explore the possible underlying mechanisms.
RESULTS
In this study, post-treatment with HSE resulted in dose-dependent decreases in both the RNA levels and TCID of SARS-CoV-2 in Vero E6 cells; treatment with 50 μg/ml and 100 μg/ml alcohol HSEs achieved up to 95.323% and 92.587% inhibition, respectively. Moreover, pre-treatment with aqueous HSEs effectively reduced the RNA levels, and TCID of SARS-CoV-2 by up to 99.684%, and alcohol HSEs achieved up to 99.921% inhibition; both of these effects occurred in a dose-dependent manner.
CONCLUSION
The results suggest that HSEs may have the potential to prevent SARS-CoV-2 infection.
背景
在当前的2019冠状病毒病（COVID-19）时代，我们有兴趣寻找除了目前可用的抗病毒药物帕罗韦德和莫努匹韦之外的药物，这些药物副作用最小且不会对人体造成伤害。金银花提取物（HSE）是一种传统中药，在其他研究中已显示出具有抗病毒作用。然而，尚无研究表明HSE对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）是否具有抑制作用。
方法
我们从干金银花中制备了HSE。我们进行了细胞活力测定、半数组织培养感染剂量（TCID）测定和定量逆转录聚合酶链反应（qRT-PCR），并使用里德-孟奇方法计算病毒滴度，以评估水提和醇提HSE对SARS-CoV-2的抑制作用，并探索可能的潜在机制。
结果
在本研究中，用HSE处理后，Vero E6细胞中SARS-CoV-2的RNA水平和TCID均呈剂量依赖性下降；用50μg/ml和100μg/ml醇提HSE处理分别实现了高达95.323%和92.587%的抑制率。此外，用水提HSE预处理可有效降低SARS-CoV-2的RNA水平和TCID，抑制率高达99.684%，醇提HSE的抑制率高达99.921%；这两种作用均呈剂量依赖性。
结论
结果表明，HSE可能具有预防SARS-CoV-2感染的潜力。
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