不同死亡诱导水平下Wistar大鼠脑死亡的诊断

Diagnosis of brain death in wistar rats at different levels of death induction.

作者信息

Gil Mazzante Nayara Maria, Zuliani Fernanda, de Oliveira Rogerio Antonio, Soares Bodaneze Júlia, Farina Panebianco Giovanna, Freitas de Souza Natália, Carmona Dinau Fernando, Alejandra Montenegro Cuellar Paola, Yumi Yamamo Dos Santos Nadia, de Souza da Silva Ana Beatriz, de Freitas Alves Vieira Fernanda, Camargo Faraldo Natália, Abreu Botelho Gabriela, Barthelson Carvalho de Moura Fernanda, Sousa Rocha Noeme

机构信息

Department of Veterinary Clinics, School of Veterinary Medicine and Animal Science, São Paulo State University - Botucatu Campus, Botucatu, State of São Paulo, Brazil.

Department of Biostatistics, Institute of Biosciences, São Paulo State University (UNESP), Botucatu, State of São Paulo, Brazil.

出版信息

F1000Res. 2025 May 6;13:1490. doi: 10.12688/f1000research.157233.2. eCollection 2024.

DOI:10.12688/f1000research.157233.2

PMID:40385977

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12082075/

Abstract

OBJECTIVE

This study aimed to evaluate hematologic, biochemical, and gasometric parameters in Wistar rats to better understand brain death parameters and reduce early misdiagnoses.

METHODS

Fifteen adult male Wistar rats (Rattus norvergicus; HanUnib: WH) were randomly distributed into three groups of five animals: the control group (G0) with evaluation performed before brain death, and two groups (G1 and G2) with brain death induced at different times: immediately after induction (G1) and one hour after induction (G2). Venous and arterial blood samples were taken to perform complete blood count, biochemical, and blood gas assays. Samples were taken at specific times based on the group each rat belonged to.

RESULTS

Statistically significant mean values were observed (P<0,05) for segmented cells (G1>G2 and G0>G2), monocytes (G2>G1 and G0>G1), creatinine (G2>G0), aspartate aminotransferase (G1>G0), potassium (G2>G0), and bicarbonate (G0>G1).

DISCUSSION

Furthermore, brain death showed a unique response in each organism, complicating its precise determination even more.

摘要

目的

本研究旨在评估Wistar大鼠的血液学、生化和气体测量参数，以更好地理解脑死亡参数并减少早期误诊。

方法

将15只成年雄性Wistar大鼠（褐家鼠；HanUnib: WH）随机分为三组，每组五只动物：对照组（G0）在脑死亡前进行评估，另外两组（G1和G2）在不同时间诱导脑死亡：诱导后立即（G1）和诱导后一小时（G2）。采集静脉和动脉血样本进行全血细胞计数、生化和血气分析。根据每只大鼠所属的组在特定时间采集样本。