Hinkley R E, Wright B D
J Exp Zool. 1985 Mar;233(3):473-7. doi: 10.1002/jez.1402330316.
We have developed a procedure for isolating intact sperm asters in quantity from fertilized sea urchin eggs. This procedure is based on detergent-extraction methods developed previously for the bulk isolation of mitotic apparatuses. Using this protocol it is possible to isolate sperm asters as soon as they appear in the fertilized egg or at any subsequent point in their brief existence.
我们已经开发出一种从受精海胆卵中大量分离完整精子星体的方法。该方法基于先前为大量分离有丝分裂装置而开发的去污剂提取方法。使用此方案，一旦精子星体出现在受精卵中或在其短暂存在的任何后续阶段，就有可能将其分离出来。
