Liu Menglan, Su Zhaoran, Hagemann Paul-Philipp, Fischer Malte, Linnebacher Michael
Molecular Oncology and Immunotherapy, Clinic of General Surgery, University Medical Center Rostock, Rostock, Germany.
Department of Gastrointestinal Surgery, People's Hospital of Tongling City, Tongling, China.
Cancer Med. 2025 May;14(10):e70976. doi: 10.1002/cam4.70976.
Colorectal cancer (CRC) is one of the most prevalent malignancies worldwide. Tumor Treating Fields (TTFields), a novel anticancer therapy using intermediate frequency and low-intensity alternating electric fields, has demonstrated efficacy in various cancers, but its application in CRC remains underexplored.
To determine the optimal frequency and treatment duration of TTFields therapy for CRC through in vitro experiments.
Four human CRC cell lines (2 MSI: HROC110 T1 M7, HROC285 T0 M2; 2 MSS: HROC450, HROC463) were treated using the inovitro TTFields system. Frequencies (100, 200, 300 kHz) and daily exposure durations (16, 20, 24 h/day) were varied. Cell viability was assessed after 72 h using crystal violet staining and spectrophotometry. Data were analyzed using t-tests or ANOVA, with p < 0.05 considered statistically significant.
TTFields significantly reduced cell viability at all tested frequencies. The 100 kHz frequency yielded the most pronounced effects, especially in MSI cell lines (p < 0.001). Treatment duration of 24 h/day led to the greatest viability reduction across all cell lines (p < 0.01), while 16 h/day and 20 h/day showed comparatively weaker effects.
Our study demonstrates that TTFields effectively reduce CRC cell viability, supporting their therapeutic potential. Further studies are needed to understand their mechanisms and synergy with existing treatments.
结直肠癌（CRC）是全球最常见的恶性肿瘤之一。肿瘤治疗电场（TTFields）是一种使用中频和低强度交变电场的新型抗癌疗法，已在多种癌症中显示出疗效，但其在结直肠癌中的应用仍未得到充分探索。
通过体外实验确定TTFields疗法治疗结直肠癌的最佳频率和治疗持续时间。
使用体外TTFields系统处理四种人结直肠癌细胞系（2种微卫星高度不稳定：HROC110 T1 M7、HROC285 T0 M2；2种微卫星稳定：HROC450、HROC463）。改变频率（100、200、300kHz）和每日暴露持续时间（16、20、24小时/天）。72小时后使用结晶紫染色和分光光度法评估细胞活力。使用t检验或方差分析对数据进行分析，p<0.05被认为具有统计学意义。
在所有测试频率下，TTFields均显著降低细胞活力。100kHz频率产生的效果最为显著，尤其是在微卫星高度不稳定细胞系中（p<0.001）。每天24小时的治疗持续时间导致所有细胞系的活力降低最大（p<0.01），而每天16小时和20小时的效果相对较弱。
我们的研究表明，TTFields可有效降低结直肠癌细胞活力，支持其治疗潜力。需要进一步研究以了解其机制以及与现有治疗方法的协同作用。
