Méndez Valentina, Sepúlveda Mario, Izquierdo-Fiallo Katherin, Macaya Constanza C, Esparza Teresa, Báez-Matus Ximena, Durán Roberto E, Levicán Gloria, Seeger Michael
Molecular Microbiology and Environmental Biotechnology, Department of Chemistry & Center of Biotechnology Daniel Alkalay Lowitt, Universidad Técnica Federico Santa María, Avenida España 1680, 2390123 Valparaíso, Chile.
Millennium Nucleus Bioproducts, Genomics and Environmental Microbiology (BioGEM), Avenida España 1680, 2390123 Valparaíso, Chile.
FEMS Microbiol Rev. 2025 Jan 14;49. doi: 10.1093/femsre/fuaf021.
The adaptive mechanisms of Burkholderiales during the catabolism of aromatic compounds and abiotic stress are crucial for their fitness and performance. The aims of this report are to review the bacterial adaptation mechanisms to aromatic compounds, oxidative stress, and environmental stressful conditions, focusing on the model aromatic-degrading Paraburkholderia xenovorans LB400, other Burkholderiales, and relevant degrading bacteria. These mechanisms include (i) the stress response during aromatic degradation, (ii) the oxidative stress response to aromatic compounds, (iii) the metabolic adaptation to oxidative stress, (iv) the osmoadaptation to saline stress, (v) the synthesis of siderophore during iron limitation, (vi) the proteostasis network, which plays a crucial role in cellular function maintenance, and (vii) the modification of cellular membranes, morphology, and bacterial lifestyle. Remarkably, we include, for the first time, novel genomic analyses on proteostasis networks, carbon metabolism modulation, and the synthesis of stress-related molecules in P. xenovorans. We analyzed these metabolic features in silico to gain insights into the adaptive strategies of P. xenovorans to challenging environmental conditions. Understanding how to enhance bacterial stress responses can lead to the selection of more robust strains capable of thriving in polluted environments, which is critical for improving biodegradation and bioremediation strategies.
伯克霍尔德氏菌目在芳香族化合物分解代谢和非生物胁迫过程中的适应性机制对其适应性和性能至关重要。本报告的目的是综述细菌对芳香族化合物、氧化应激和环境胁迫条件的适应机制，重点关注模式芳香族降解菌嗜麦芽窄食单胞菌LB400、其他伯克霍尔德氏菌目细菌以及相关降解细菌。这些机制包括：（i）芳香族降解过程中的应激反应；（ii）对芳香族化合物的氧化应激反应；（iii）对氧化应激的代谢适应；（iv）对盐胁迫的渗透适应；（v）铁限制期间铁载体的合成；（vi）蛋白质稳态网络，其在维持细胞功能中起关键作用；（vii）细胞膜、形态和细菌生活方式的改变。值得注意的是，我们首次纳入了对嗜麦芽窄食单胞菌蛋白质稳态网络、碳代谢调节和应激相关分子合成的新基因组分析。我们通过计算机模拟分析这些代谢特征，以深入了解嗜麦芽窄食单胞菌对具有挑战性的环境条件的适应策略。了解如何增强细菌的应激反应可导致选择出能够在污染环境中茁壮成长的更强壮菌株，这对于改进生物降解和生物修复策略至关重要。
