Zhao Xu, Zhao Jianwen, Cui Chun
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, Guangdong, China.
Food Funct. 2025 Jun 3;16(11):4562-4574. doi: 10.1039/d5fo00090d.
Sugarcane molasses, a by-product of the sugar manufacturing process, is rich in polyphenols and possesses potential physiological activities. This study aimed to extract and purify polyphenols from sugarcane molasses and assess their hypoglycemic activity through α-glucosidase inhibition and intracellular glucose transport inhibition assays. Polyphenols were extracted using resin adsorption and further purified ultrafiltration, yielding a 23.07% polyphenol content. Thirty phenolic compounds were identified by HPLC-MS/MS. The IC value of sugarcane polyphenols for α-glucosidase inhibition is 114.1366 mg mL. Molecular docking revealed that polyphenols interact with α-glucosidase primarily through hydrogen bonding, with key binding sites at Gln256, Asp327, Arg411 and Phe163. Additionally, evaluation of a Caco-2 cell transmembrane transport model showed that sugarcane polyphenols significantly inhibited glucose transmembrane transport and downregulated the mRNA expression of SGLT1, GLUT2, glucosidase, and sodium-potassium ATPase. These findings suggest that sugarcane molasses is a promising source of polyphenols with potential hypoglycemic activity and provide valuable insights into the mechanism of α-glucosidase and glucose transport inhibition.
甘蔗 molasses 是制糖过程的一种副产品，富含多酚且具有潜在的生理活性。本研究旨在从甘蔗 molasses 中提取和纯化多酚，并通过α-葡萄糖苷酶抑制和细胞内葡萄糖转运抑制试验评估其降血糖活性。采用树脂吸附法提取多酚，再通过超滤进一步纯化，得到多酚含量为 23.07%。通过 HPLC-MS/MS 鉴定出 30 种酚类化合物。甘蔗多酚对α-葡萄糖苷酶抑制的 IC 值为 114.1366 mg/mL。分子对接显示，多酚主要通过氢键与α-葡萄糖苷酶相互作用，关键结合位点在 Gln256、Asp327、Arg411 和 Phe163。此外，对 Caco-2 细胞跨膜转运模型的评估表明，甘蔗多酚显著抑制葡萄糖跨膜转运，并下调 SGLT1、GLUT2、葡萄糖苷酶和钠钾 ATP 酶的 mRNA 表达。这些发现表明，甘蔗 molasses 是一种有潜力的多酚来源，具有潜在的降血糖活性，并为α-葡萄糖苷酶和葡萄糖转运抑制机制提供了有价值的见解。
