Opsin gene expression plasticity and spectral sensitivity in male damselflies could mediate female colour morph detection.

The visual systems of Odonata are characterized by many opsin genes, which form the primary light-sensitive photopigments of the eye. Female-limited colour polymorphisms are also common in Odonata, with one morph typically exhibiting male-like (androchrome) coloration and one or two morphs exhibiting female-specific coloration (gynochromes). These colour polymorphisms are thought to be maintained by frequency-dependent sexual conflict, in which males form search images for certain morphs, causing disproportionate mating harassment. Here, we investigate opsin sensitivity and gene expression plasticity in mate-searching males of the damselfly during adult maturation and across populations with different female morph frequencies. We find evidence for opsin-specific plasticity in relative and proportional opsin mRNA expression, suggesting changes in opsin regulation and visual sensitivity during sexual maturation. In particular, expression of the long-wavelength-sensitive opsin LWF2 changed over development and varied between populations with different female morph frequencies. UV-Vis analyses indicate that short- and long-wavelength opsins absorb wavelengths of light between 350 and 650 nm. Assuming opponency between photoreceptors with distinct short- and long-wavelength sensitivities, these sensitivities suggest male spectral visual discrimination ability of androchrome and gynochrome females. Overall, our results suggest that opsin sensitivity and expression changes contribute to visual tuning that could impact conspecific discrimination.