Henry M F, Bonner W D, Nyns E J
Biochim Biophys Acta. 1977 Apr 11;460(1):94-100. doi: 10.1016/0005-2728(77)90155-4.
The involvement of iron in the biogenesis of the cyanide-insensitive respiration in the yeast Saccharomycopsis lipolytica has been established on the following basis: (1) endogenous metal chelation by either benzyl- or salicylhydroxamic acid, EDTA or nitrilotriacetate prevented the biogenesis of the cyanide-insensitive respiratory pathway in S. lipolytica. (2) Addition of Fe(III) during the biogenesis increased both the rate of the appearance of the alternative respiratory pathway and its extent. Neither Fe(II), nor Co(II), Cu(II), Al(III), La(III), Mn(II) or Mg(II) could substitute for Fe(III). (3) The biogenesis of the alternative respiratory pathway could be dissociated into two steps: (a) a first one, slow, cycloheximide-sensitive, temperature-dependent, iron-independent, leading to cells still fully cyanide-sensitive, presumably involving the de novo biosynthesis of an inactive protein moiety and (b) a second step, fast, iron-dependent, temperature-independent, cycloheximide-insensitive, leading to cells with a cyanide-insensitive respiration, presumably the activation by iron of the inactive precursor.
铁参与解脂耶氏酵母（Saccharomycopsis lipolytica）中氰化物不敏感呼吸的生物合成已基于以下依据得以确立：（1）苄基或水杨基异羟肟酸、乙二胺四乙酸（EDTA）或次氮基三乙酸进行的内源性金属螯合可阻止解脂耶氏酵母中氰化物不敏感呼吸途径的生物合成。（2）在生物合成过程中添加铁（III）可提高替代呼吸途径出现的速率及其程度。亚铁（II）、钴（II）、铜（II）、铝（III）、镧（III）、锰（II）或镁（II）均不能替代铁（III）。（3）替代呼吸途径的生物合成可分为两个步骤：（a）第一步，缓慢，对放线菌酮敏感，依赖温度，不依赖铁，导致细胞仍对氰化物完全敏感，推测涉及无活性蛋白质部分的从头生物合成；（b）第二步，快速，依赖铁，不依赖温度，对放线菌酮不敏感，导致细胞具有氰化物不敏感呼吸，推测是铁对无活性前体的激活。
