Chen Wei, Zhang Zhao, Zhang Xiao, Zuo Jing, Li Xiao, Li Shuang-Cheng, Gao Yue-Ming
Department of Rehabilitation Medicine, The Second Medical Center and National Clinical Research Center for Geriatric Diseases, Chinese PLA General Hospital, Beijing, China.
College of PLA Medicine, Beijing, China.
Lasers Surg Med. 2025 May 20. doi: 10.1002/lsm.70030.
254 nm short wave ultraviolet (UVC) emitted by low-pressure mercury lamp is effective in the treatment of skin wounds. With the emergence of deep ultraviolet light-emitting diodes (LED), it is expected to significantly expand the clinical indications of UVC. However, whether 254 nm UVC generated by LED light source can promote skin wound healing has not been reported.
To clarify the effect of 254 nm UVC-LED light source on promoting skin wound healing and explore the mechanism of UVC-LED promoting wound healing.
The full-thickness wound model of rat back skin was constructed, and the skin wounds of rats were treated with low-pressure mercury lamp and 254 nm UVC-LED equipment respectively. The curative effect of UVC-LED was determined by wound healing rate and histopathological changes. The expression of transforming growth factor-alpha (TGF-α), epidermal growth factor receptor (EGFR), cluster of differentiation 31 (CD31), cluster of differentiation 68 (CD68), vascular endothelial growth factor (VEGF) and the mRNA content of TGF-α, EGFR, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and VEGF were detected to clarify the mechanism of UVC-LED promoting wound healing.
Histopathology showed that both low-pressure mercury lamp and UVC-LED irradiation could promote skin wound healing, reduce the degree of inflammatory response, and promote angiogenesis. UVC-LED could better promote collagen fiber proliferation. UVC-LED promotes the expression of key cytokines in wound re-epithelialization by increasing the levels of TGF-α and EGFR, dynamically regulates wound healing by bidirectionally regulating the expression of inflammatory factors TNF-α and IL-6, promotes wound angiogenesis by increasing the levels of CD31 and VEGF, and reduces and regulates inflammation by increasing the number of macrophages by increasing CD68.
254 nm UVC-LED can significantly promote skin wound healing in rats and play a role by regulating tissue regeneration cytokines, inflammatory factors and angiogenesis mechanisms.
低压汞灯发出的254纳米短波紫外线（UVC）对皮肤伤口治疗有效。随着深紫外发光二极管（LED）的出现，有望显著扩大UVC的临床应用范围。然而，LED光源产生的254纳米UVC是否能促进皮肤伤口愈合尚未见报道。
阐明254纳米UVC-LED光源对促进皮肤伤口愈合的作用，并探讨UVC-LED促进伤口愈合的机制。
构建大鼠背部皮肤全层伤口模型，分别用低压汞灯和254纳米UVC-LED设备处理大鼠皮肤伤口。通过伤口愈合率和组织病理学变化确定UVC-LED的疗效。检测转化生长因子-α（TGF-α）、表皮生长因子受体（EGFR）、分化簇31（CD31）、分化簇68（CD68）、血管内皮生长因子（VEGF）的表达以及TGF-α、EGFR、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和VEGF的mRNA含量，以阐明UVC-LED促进伤口愈合的机制。
组织病理学显示，低压汞灯和UVC-LED照射均能促进皮肤伤口愈合，减轻炎症反应程度，促进血管生成。UVC-LED能更好地促进胶原纤维增殖。UVC-LED通过提高TGF-α和EGFR水平促进伤口再上皮化关键细胞因子的表达，通过双向调节炎症因子TNF-α和IL-6的表达动态调节伤口愈合，通过提高CD31和VEGF水平促进伤口血管生成，通过增加CD68提高巨噬细胞数量减轻和调节炎症。
254纳米UVC-LED能显著促进大鼠皮肤伤口愈合，并通过调节组织再生细胞因子、炎症因子和血管生成机制发挥作用。
