Jin Xin, Zhao Qingqing, Hao Xiaowen, Shi Peicong, Wang Yan, Wang Pei
Department of Oncology, Xuzhou Central Hospital, Xuzhou, China.
Department of Breast Surgery, Xuzhou Central Hospital, Xuzhou, China.
IUBMB Life. 2025 May;77(5):e70019. doi: 10.1002/iub.70019.
Proline- and serine-rich coiled-coil 1 (PSRC1) has been implicated in various cancers, yet its role in breast cancer (BRCA) remains incompletely understood. Here, we employed the UALCAN database to explore PSRC1 expression in BRCA and obtained survival prognosis data from the Kaplan-Meier Plotter database. Additionally, PSRC1 expression was analyzed in 81 pairs of BRCA tissues and their corresponding adjacent noncancerous tissues through quantitative real-time PCR, western blotting, and immunohistochemistry. We observed PSRC1 was overexpressed in BRCA tissues, especially in triple negative breast cancer (TNBC). Higher PSRC1 levels correlated with poorer outcomes for BRCA patients. In 81 BRCA tumor tissues, PSRC1 protein levels were significantly associated with positive vessel tumor embolus. Subsequently, the clinical relevance of PSRC1 in BRCA was assessed using the chi-square test, the Kaplan-Meier model with a Log-rank test, as well as univariate and multivariate analyses. Patients with high PSRC1 had worse prognoses. Elevated PSRC1 expression served as an independent predictor of prognosis. Moreover, we investigated the effects of PSRC1 on BRCA cell phenotypes in MCF-7 and BT549 cells and used a mouse xenograft model with BT549 cells to determine its in vivo effect. Both in vitro and in vivo experiments demonstrated that silencing PSRC1 inhibited cell proliferation, migration, and tumor development. In summary, our results indicate that high PSRC1 expression is closely linked to BRCA patient survival and could be a valuable prognostic biomarker for this disease.
富含脯氨酸和丝氨酸的卷曲螺旋蛋白1（PSRC1）与多种癌症有关，但其在乳腺癌（BRCA）中的作用仍未完全明确。在此，我们利用UALCAN数据库探究BRCA中PSRC1的表达情况，并从Kaplan-Meier Plotter数据库获取生存预后数据。此外，通过定量实时PCR、蛋白质免疫印迹法和免疫组织化学，对81对BRCA组织及其相应的癌旁非癌组织中的PSRC1表达进行了分析。我们观察到PSRC1在BRCA组织中过表达，尤其是在三阴性乳腺癌（TNBC）中。较高的PSRC1水平与BRCA患者较差的预后相关。在81例BRCA肿瘤组织中，PSRC1蛋白水平与血管肿瘤栓子阳性显著相关。随后，使用卡方检验、带有对数秩检验的Kaplan-Meier模型以及单因素和多因素分析评估了PSRC1在BRCA中的临床相关性。PSRC1水平高的患者预后较差。PSRC1表达升高是预后的独立预测因素。此外，我们研究了PSRC1对MCF-7和BT549细胞中BRCA细胞表型的影响，并使用BT549细胞的小鼠异种移植模型来确定其体内作用。体外和体内实验均表明，沉默PSRC1可抑制细胞增殖、迁移和肿瘤发展。总之，我们的结果表明，高PSRC1表达与BRCA患者的生存密切相关，可能是该疾病有价值的预后生物标志物。
