Alrumaih Sara, Alshibani Nouf, Khounganian Rita, Alshehri Fahad A, Allam Eman, Alkattan Reem
Department of Periodontics and Community Dentistry, College of Dentistry, King Saud University, Riyadh, Saudi Arabia.
Hail Health Cluster, Ministry of Health, Hail, Saudi Arabia.
Saudi Dent J. 2025 Apr 14;37(1-3):2. doi: 10.1007/s44445-025-00003-4.
Bone loss, linked with numerous oral conditions such as periodontal diseases and periimplantitis, poses a significant challenge for dental clinicians. The current study evaluated the in vivo effects of local application of Resolvin E1 (RvE1) on bone regeneration in critical size calvarial defects in rats. Thirty female Wistar rats with 5 mm induced calvarial defects were randomly allocated into four groups: no treatment (negative control, n = 5), treatment using bovine bone grafts (positive control, n = 5), treatment using local delivery of RvE1 (n = 11) and treatment using RvE1 mixed with bovine bone graft (n = 9). After 12 weeks, the animals were sacrificed and the calvarial defects with the adjacent tissues were sectioned en-bloc and prepared for histological examination. A comprehensive qualitative and quantitative histological examination was performed to assess bone regeneration and its relation to the defect, the presence of remnant bone and RvE1 particles, the integration of the native bone with the newly formed bone, bone density and bony trabeculae, the inflammatory reaction, the connective tissue bridging in the defect, and the encapsulating fibrous tissue. Signs of neovascularization, increased cellularity, lack of the organized lamellated appearance of mature bone, disorganized arrangement of osteocytes, osteoblasts and osteoclasts were also assessed. Comparisons of the quantitative values between all studied groups indicated statistically significant differences (p ≤ 0.05) in all parameters except for the increased cellularity and granulation tissue. Histological findings indicate that RvE1 with adjunct bone graft significantly enhanced the bone formation compared to RvE1 or bovine graft alone.
骨质流失与多种口腔疾病如牙周病和种植体周围炎相关，给牙科临床医生带来了重大挑战。本研究评估了局部应用消退素E1（RvE1）对大鼠临界大小颅骨缺损骨再生的体内影响。将30只诱导形成5毫米颅骨缺损的雌性Wistar大鼠随机分为四组：不治疗（阴性对照组，n = 5）、使用牛骨移植治疗（阳性对照组，n = 5）、使用局部递送RvE1治疗（n = 11）和使用RvE1与牛骨移植混合治疗（n = 9）。12周后，处死动物，将带有相邻组织的颅骨缺损整块切片，准备进行组织学检查。进行了全面的定性和定量组织学检查，以评估骨再生及其与缺损的关系、残余骨和RvE1颗粒的存在、天然骨与新形成骨的整合、骨密度和骨小梁、炎症反应、缺损处的结缔组织桥接以及包封纤维组织。还评估了新生血管形成的迹象、细胞增多、缺乏成熟骨有组织的板层外观、骨细胞、成骨细胞和破骨细胞的无序排列。所有研究组之间定量值的比较表明，除细胞增多和肉芽组织外，所有参数均存在统计学显著差异（p≤0.05）。组织学结果表明，与单独使用RvE1或牛骨移植相比，RvE1联合骨移植显著增强了骨形成。
