早期小鼠胚胎和滋养层组织外植体的3D体外培养

3D In Vitro Culture of Early Mouse Embryos and Trophoblast Tissue Explants.

作者信息

Prit Devila, Jaitly Shashank, Govindasamy Niraimathi, Ranga Adrian, Bedzhov Ivan

机构信息

Embryonic Self-Organization Research Group, Max Planck Institute for Molecular Biomedicine, Münster, Germany.

Laboratory of Bioengineering and Morphogenesis, Department of Mechanical Engineering, KU Leuven, Leuven, Belgium.

出版信息

Methods Mol Biol. 2025 May 22. doi: 10.1007/7651_2025_646.

DOI:10.1007/7651_2025_646

PMID:40397278

Abstract

The cellular dynamics during peri-implantation embryogenesis and the concurrent interactions at the embryo-maternal interface are inherently difficult to study due to intrauterine development in mammals. To model certain aspects of these processes in vitro, we have generated a biomimetic environment resembling the mechanical properties of the murine uterine stroma. Here we describe a step-by-step methodology for 3D culture of mouse embryos and ectoplacental cone explants in synthetic hydrogels that allow ex utero trophoblast invasion.

摘要

由于哺乳动物的胚胎在子宫内发育，植入前胚胎发生过程中的细胞动力学以及胚胎与母体界面处同时发生的相互作用本质上很难研究。为了在体外模拟这些过程的某些方面，我们构建了一种类似于小鼠子宫基质力学特性的仿生环境。在此，我们描述了一种在合成水凝胶中对小鼠胚胎和外胎盘锥外植体进行三维培养的分步方法，该方法可使滋养层在子宫外进行侵袭。