Sacks D L, Perkins P V
Am J Trop Med Hyg. 1985 May;34(3):456-9. doi: 10.4269/ajtmh.1985.34.456.
Midgut promastigotes were obtained from Phlebotomus papatasi and Lutzomyia longipalpis on days 3-7 after infection with cloned isolates of Leishmania major and Leishmania mexicana amazonensis, respectively, and examined as to their ability to initiate cutaneous infections in BALB/c mice. Sequential development of midgut promastigotes from a noninfective to an infective stage was confirmed for both the New World and Old World species. The generation of infective promastigotes from rapidly dividing avirulent populations occurred as early as day 3 and was well under way by day 4 after infective feed. Optimally infective promastigotes were recovered from midguts shortly after bloodmeal passage, coinciding with the time at which another bloodmeal is sought by the fly.
分别在感染利什曼原虫主要亚种和亚马逊利什曼原虫克隆分离株后的第3至7天，从巴氏白蛉和长须罗蛉中获取中肠前鞭毛体，并检测它们在BALB/c小鼠中引发皮肤感染的能力。对于新大陆和旧大陆的物种，均证实了中肠前鞭毛体从非感染阶段到感染阶段的连续发育过程。从快速分裂的无毒群体中产生感染性前鞭毛体最早在感染性进食后的第3天出现，到第4天已充分发展。在血餐通过后不久，从中肠中回收了最佳感染性的前鞭毛体，这与苍蝇寻找另一顿血餐的时间相吻合。
